Dr. Christina Godfried Sie

• Molecular Operations product development of diagnostics or IVDs (RUOs, LDTs, and companion diagnostics) in the liquid biopsies oncology field • Whole genome amplification of rare Circulating Tumor Cell DNA for Next-Generation Sequencing offerings • Isolation and analysis of cell-free DNA • Molecular testing services (qRT-PCR)

• Established CRISPR technology capability within six months • CRISPR team tech lead scientist on cell line characterization • Performing genome editing techniques to develop clinically relevant clonal cell lines with specific genome alterations or reporter functions for target pathway validation • Characterizing cell lines using molecular biology techniques and cellular assays • Improving genome editing protocols of CRISPR to increase efficiency and reduce off-target effects

Transcription and translation of patient/doctor interactions from audio files.

• Designed and implemented experiments to examine functions of cohesins in genome maintenance and organization using the model organism Saccharomyces cerevisiae • Performed and evaluated backcross experiments using micromanipulators and microscopy to purify heterogenic yeast strains for whole genome sequencing • Managed and analyzed large datasets in Microsoft Excel and interpreted results

• Prepared presentations and taught recitations of “Cellular and Molecular Biology”, and “Genetics” introductory classes • Guestlecturer on “Cellular Cytoskeleton” • Devised multiple-choice test questions for quarterly and final exams • Led group of undergraduates to identify post-transcriptional recoding events in the human transcriptome using bioinformatics and molecular biology techniques • Managed preparation of research lab course to isolate and characterize Mycobacteria Phages

-Identified A-to-I RNA editing targets by genome-wide biocomputational screening using the UCSC Genome Browser, mfold, BLAST -Verified editing targets with molecular techniques like PCR, sequencing and cloning -Assessed impact of editing on transcript maturation and protein function using tools like cloning, site-directed mutagenesis, qPCR, transfection of cell lines -Analyzed recombinant protein isoforms using biochemical tools like Western blot, radioblot, in vitro transcription/translation, proteolysis

Evaluated small-colony phenotype of yeast expressing BRCA1-fragments in a strain lacking a genome maintenance gene by microscopy and FACS analysis (Skibbens 2008) | Cloned deletion mutants of a gene and verified expression of fragments by Western blot | Purified high expressers of HeLas stably transfected with fluorescently labeled gap junctions | Pilot tested microscopy studies of mammalian cells proliferating on glass biomaterial

Assisted first press conference of Arena GmbH on their broadcast rights of the Bundesliga | Arranged meetings and travel itineraries, implemented filing system

Responsible for human tissue samples, culture of primary mammalian cells | Developed protocols to engineer articular cartilage to create autologous joint implants, supervised lab technician and interns | Planned and managed animal pilot study to evaluate repair strategy for osteochondral defects based on tissue engineered grafts | Part of team to develop automated cell culture system | Led collaboration with academic group to assess impact of growth factors on clinical-scale expansion of primary cells

Identified Mycobacteria species in fecal samples of livestock with Tuberculosis and in Crohn’s patients by multiplex PCR

Advanced Cell Biology | Eukaryotic Biochemistry | Principles of Protein Structure | Molecular Genetics | Elements Biochemistry II | Molecular Cell Bio I | Molecular Cell Bio II | Structure and Function of RNAs and Ribonucleoprotein Complexes | Techniques and Research Strategies in Molecular Genetics

Biochemie | Mikrobiologie | Zellbiologie | Genetik | Pflanzenphysiologie