Jasmin Freytag

Structural characterization of Polyomavirus Receptor Interactions and Cell Entry Inhibition

Structural studies of bacterial metabolic enzymes Methods: Protein expression and purification, lysis buffer screens, IMAC, SEC, SDS-PAGE, crystallization, data acquisition, structure refinement

Structural studies of nuclear mRNA-protein complexes Methods: Recombinant expression, purification and characterisation of endogenous proteins from eukaryotic cell lines; negative stain of mRNA-protein complexes

Structural analysis of human Polyoma Viruses Methods: Protein expression and purification, lysis buffer screens, IMAC, SEC, SDS-PAGE, surface plasmon resonance (SPR), crystallization, data acquisition, structure refinement

Supervision of first semester students in their basic practical course, including seminars Topics: acids/bases, titration curves, complex formation, basic thermodynamics and kinetics, ester bond formation, chromatography techniques, lipid composition and distribution, lipid extraction from tissue

Expression and localization of Potassium channels in eukaryotic cell culture Methods: PCR, Gel electrophoresis, Eukaryotic cell culture, Western Blot, Transfection, Live-cell imaging, Fluorescence microscopy, Image analysis