Miriam Ender

Methods performed: antibiotic MIC determinations

general PCR

iPCR; Southern-

Northern-

Westernblot

protein expression in E. coli for electro mobility

protein expression in Pichia pastoris

knock out mutants

cloning of complementation plasmids

site directed mutagenesis

transposon library

sequencing

pulsed fieled gel electrophoresis

continuous cultures

reporter fusion; primer extension; HPLC

Capillare electrophoresis

chromatography

ELISA

Atomic force microscopy

kinetic measurmets using Octed red (Forté bio)

eucaryotic cell culture handling

to read

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