Niharika Garg

Analyzed complex datasets to identify inefficiencies and propose data-driven solutions. Developed predictive models to optimize processes, aligning with operational goals, while collaborating in agile teams. Skills:- Agile framework, Teamwork, Project Management, Python, SQL, Supervised and Unsupervised ML, ComputerVision, NLP, Webscrapping, TensorFlow, Keras, PyTorch, Scikit-learn, Matplotlib and Seaborn

• Investigated the role of cell cytoskeleton machinery ‘actin-myosin’ in development of the unique phylum distinctive cell type. • Lab techniques- immunofluorescence, Western blots, siRNA (electroporation), Recombinant Protein expression Mammalian and Bacterial cell culture, high-resolution confocal fluorescence microcopy, bright field and DIC microscopy. • Software - Image J and Python libraries to analyse the microscopy data

Gained experience clean room, Fabrication of microfluidic chips through soft lithography, production of hydrogel beads through droplet microfluidics, Real Time deformability Cytometry.

To study the contractile nature in detail and find out the reason behind its mechanics. Also, we would like to study the cortical flows and the pseudo cleavage due to series of contractions mainly due to actin cytoskeleton and cytoplasmic flows. We would also explore genetics in C. Elegans since it has complete genetic sequence and therefore serves as good model organism to do eukaryotic genetic studies.

A short term Lab project on "Investigation of the novel conductive continuous phase in droplet microfluidics".

Short term project on "Roles of somatic cells contractility in Caenorhabditis elegans germline cytoplasmic streaming".

Worked on Synthetic biology tools in order to analyse RNA-lipid interactions. Worked on synthesis of membranes by lipids or fatty acids and analysing their stability at various conditions such as differnt pH , temperature, Salt concentration in order to find the most optimum conditions. Finally, the binding assay experiments were done in order to study the interaction of these membranes with RNA.

Studied the development of Nematocyst (stinging cell) using ‘Hydra’ as model organism. • Lab techniques - immunofluorescence, Western blots, siRNA through electroporation, Recombinant Protein expression Mammalian and Bacterial cell culture, high-resolution confocal fluorescence microcopy, Immuno-Electron Microscopy, bright field and DIC microscopy. • Software tools - Image J and Python using common libraries like NumPy, pandas and Matplotlib to analyse the microscopy data.

Lab courses: Atomic Force Microscopy, Scanning Tunneling microscopy, Scanning Electron Microscopy, Single-molecule FRET, Protein Purification and expression´, Western blotting. Course work: Cellular Machines, Biochemistry, Theoretical Biophysics, Biological Hydrodynamics, Molecular Nanostructures, Bioinspired devices, Molecular cell Biology.

Biochemistry, Biophysics, Bioinformatics, Molecular Biology, Thermodynamics, Pharmaceutical biology