Nils Runge

Master thesis " Systematic determination of relevant process parameters to design a P. pastoris cultivation in Fed-batch mode for the production of a *desired protein" Media testing | Using the Respiratory Activity Monitoring System (RAMOS) | Fermentation: Develpoment of a feeding-strategy for Fed-batch stirred-tank reactor | Continoues fermentation | Analytics: on- and offline analysis, microscopy, SDS-PAGE, HPLC, GPC, Protease test-kit | Scientific evaluation and presentation

Production of liposomal food additives (5 - 120 L) | Manufacturing under quality guidelines (ISO 22000) | Process development for sample production | Consultant at product development

Production of adherent eucaryotic cells in different scales | Freezing of large batches (up to 300 vials) Qualitycontrol: immunocytical stainings, cytochrome p450 assays, hygiene-monitoring Molceular biology tasks: PCR, bacterial transformation | plasmid-DNA isolation Generating of lentiviral vectors: lentiviral transduction of primary cells | generating recombinant lentiviruses

Internship at the working group "High throughput Histology" and bachelor thesis „Charakterisierung von Synapsen und dendritischen Spines in wildtyp und krankheitsspezifischen Tiermodellen“ Production of histological sections (microtome, Cry-microtome) | Stainings of neurons and dendritic spines | Automated high throughput microscopy | Scientific evaluation and presentation

Focused on: Bioassays, Drug development, Bioprocessautomation, Biopharmaceutical engineering, Good manufacturing practice

Focused on: Measuring- and control techniques, fermentation techniques, downstream processing