Dr. Xing Xiao

-Help to establish 3D cell culture model to study dental implant-related inflammation -Extract DNA from bacteria or biofilm, and prepare samples for gene sequencing -Quantity PCR to identify bacteria composition

-Study the metabolic and behavioral regulatory roles of orexin signaling in serotonergic and dopaminergic neurons, using genetic-modified mice -Design and perform in vivo and in vitro experiments, at gene, protein, electrophysiological and behavioural levels -Substantial knowledge of brain, energy metabolism and obesity -Discovered that orexin receptors 1 and 2 in serotonergic neurons differentially regulate peripheral glucose metabolism in obesity (published in Nature Communications 2021)

-Study peripheral sensation of nociception, focusing on TRPV1 in dorsal root ganglion neurons, using rat models -Substantial hands-on experience in cell culture of primary neurons and cell lines -Design and perform experiments, including PCR, IHC, IFC, ICC, WB, co-IP, transfection, infection, cloning, E.Phys., calcium imaging, pain behaviours, etc. -Found Shp-1 dephosphorylates TRPV1 and alleviates inflammatory pain, IGF and FA up-regulate TRPV1 in cancer pain (published in 'Pain', 2015, etc)

-4 years of medicine study at classes -1 year as an intern nurse