Research assistant / Doctoral candidate / PhD student Immunology Infectious Diseases Vaccination
Research assistant / Doctoral candidate / PhD student Immunology Infectious Diseases Vaccination
Research assistant / Doctoral candidate / PhD student Immunology Infectious Diseases Vaccination
Research assistant / Doctoral candidate / PhD student Immunology Infectious Diseases Vaccination
Charité – Universitätsmedizin Berlin
Krankenhäuser
Berlin
- Art der Beschäftigung: Vollzeit
- Vor Ort
- Aktiv auf der Suche
Research assistant / Doctoral candidate / PhD student Immunology Infectious Diseases Vaccination
Über diesen Job
Salary group
Arbeiten an der Charité
The Giesecke-Thiel Lab investigates initiation, reactivation, and lifestyle of human B cell memory. This includes studying the immunological imprinting of human memory B cells from different antigen exposures, including both the nature of antigen and the anatomical site of priming. Our goal is to identify fundamental mechanisms of human immune memory and to leverage these discoveries to develop new ways to detect, prevent, and modify infection courses and outcomes.
Die Stelle im Überblick
In this project, the successful candidate will explore the phenotypic and molecular features of antigen-specific human memory B cells (mBCs) shaped by mucosal priming in comparison to systemic vaccinations. The candidate will optimize flow cytometry–based antigen-specific B cell stainings and phenotyping strategies and perform antigen-specific B cell stainings, flow cytometry analyses, and cell isolations using FACS and MACS to obtain high-quality antigen-specific populations.
A core responsibility includes establishing, benchmarking, and applying single-cell ATAC- and RNA-sequencing workflows (FLASH-seq), carried out in close collaboration with the local Core Facility. The candidate will additionally carry out basic bioinformatic analyses, including quality control, preprocessing, dimensionality reduction, and exploratory interpretation of single-cell multiomic datasets, with support from collaborating bioinformaticians.
A key task is to investigate how antigen challenges and rechallenges affect human memory B cells, thereby advancing our understanding of immune memory (re-)programming or its resilience to perturbation.
Danach suchen wir
- Solid background in immunology or molecular cell biology, with a strong interest in human B cell biology and immune memory
- Hands-on experience with flow cytometry, including multicolor panel design, data acquisition, and analysis
- Experience in cell isolation techniques, including primary human material, FACS and/or MACS, or willingness to acquire advanced sorting skills
- Basic practical or conceptual experience with single-cell sequencing technologies (scRNA-seq and/or scATAC-seq), or a strong interest in learning and applying these methods
- Ability to perform basic bioinformatic analyses of single-cell datasets, including quality control, preprocessing, and exploratory data analysis (e.g. R or Python), or strong willingness to acquire these skills
- Intellectual curiosity with a strong sense of accountability
- Strong organizational skills
- Strong communication skills
- Ability to work independently as well as collaboratively in an interdisciplinary environment involving wet-lab scientists, core facilities, and bioinformaticians
- Very good written and spoken English communication skills
Please provide a cover letter presenting your scienfici interests, why you want to do a doctorate and your goals and a curriculum vitae. Contact details of two willing referees for references. One letter of reference has to be included in the application.
Das bringt die Charité mit
- Regular working hours, an excellent working atmosphere, and a family-friendly department.
- You can expect a future-oriented, varied, and challenging role with a high degree of personal responsibility and freedom to act.
- Intercultural team events and a well-structured onboarding process with friendly colleagues are a matter of course for us.
- We offer extensive free training and continuing education opportunities, including university studies.
- Charité offers discounts on many shopping, travel, and sports activities for employees.
- In addition, there is the option of bicycle leasing through JobRad, and a bicycle clinic with discounted prices for employees on campus is available.
- We offer additional security in old age through our company pension plan.
- Certified as a family-friendly university and family-friendly company since 2007 – further information can be found here: https://familie.charite.de/
Informationen zur Stelle
- Entgeltgruppe E13 TVöD. Hier finden Sie alle Informationen zum Gehalt und Tarifvertrag
- Arbeitszeit: Teilzeitbeschäftigung in Höhe von 65 %
- Die Stelle ist im Rahmen eines Projektes ab 16.03.2026 für 3,5 Jahre befristet u.a. auch zur Qualifikation
- Bei uns sind 30 Tage Urlaub Standard
- Die Bewerbungsfrist endet am: 28.02.2026
- Kennziffer: 6549
Application
Everyone is welcome at the Charité, regardless of age, religion, gender, gender identity, sexual orientation, nationality, disability, ethnic or social background. We are committed to equal opportunities and inclusion.
Apply using our online tool and become part of the Charité.
Dr. Claudia Giesecke-Thiel, claudia.giesecke-thiel@charite.de
Data protection information
The Charité would like to point out that personal data is stored and processed at various locations at the Charité premises (e.g. specialty department, personnel representatives, HR department) as part of and for the purposes of the application process. Furthermore, the data may be transferred or processed within the group and to external bodies (e.g. authorities) to protect legitimate interests. By submitting your application, you agree to our data protection policy and terms of use for application procedures, which you can find here .
Additional information
Charité – Universitätsmedizin Berlin makes its personnel decisions based on suitability, ability and professional performance. The Charité aims to increase the proportion of women in management positions and therefore strongly encourages women to apply. Women with equivalent qualifications will be given priority within the scope of legal possibilities. Applications from people with a migration background who meet the recruitment requirements are expressly encouraged. Severely disabled applicants with equal qualifications will be given preference. A police good-conduct certificate, in some cases an extended good-conduct certificate, is required at the time of recruitment. Unfortunately, application documents can be returned only if a sufficiently stamped envelope is enclosed. Any incurred travel expenses cannot be reimbursed.
Unternehmens-Details
Charité – Universitätsmedizin Berlin
Krankenhäuser
