Your Responsibilities

The Bernecky group aims to uncover critical mechanisms of RNA regulation during and after transcription. We use purification, reconstitution, and biochemical and structural analysis (particularly cryo-EM) to characterize protein-nucleic acid complexes and investigate their function. We are looking for a self-motivated, efficient, and reliable technician to provide research support for a project in which we investigate the activity and structure of a chromatin-binding multisubunit protein complex. This will include the following tasks:

• Molecular cloning (design and preparation of plasmids)
• Protein expression in insect and mammalian cells
• Protein purification, quantification, and quality control
• Optimization and performance of biochemical and biophysical assays

Your Profile

• Masters or PhD degree in biochemistry or a related subject
• Theoretical and practical knowledge of molecular biology and protein purification, including design of purification strategies and biochemical assays
• Experience using ÄKTA chromatography systems for multi-step protein purification
• Ability to perform experiments with high reproducibility and efficiently organize your time
• Applications should include a short letter of motivation, a CV, and contact information for 2 referees