Marvin Pätz

Application development in the field of molecular biology (DNA sample preparation, primer and probe design, real-time PCR, and digital PCR)

• Development of protocols for quantification of adeno-associated viruses (AAV) of serotype 2 using qPCR and dPCR • Validation of the developed dPCR protocol for quantification of AAV2, considering regulatory requirements

• Preparation of quality and risk-related documents, including risk analyses, test plans, investigation and complaint reports • Review of manufacturing records for GMP compliance (batch record review) • Handling of deviations and complaints • Communication with customers and internal stakeholders to address customer inquiries and projects

• Proof-of-concept implementation of a time-temperature indicator for monitoring freezing times in pre-analytics • Further development of a point-of-care testing system with magnetic beads for sepsis diagnostics

• Preparation of anatomically high-quality cryosections from frozen/paraformaldehyde-fixed brain tissue • Performance of double immunofluorescence labeling on "free-floating" brain tissue sections of rats • Confocal microscopy • Quantitative analysis of colocalized cells using the ImageJ program

• Analysis of blood alcohol levels in alcohol-dependent rats • RNA extraction from rat brain tissue, cDNA generation, and qRT-PCR for gene expression analysis • In situ hybridization on rat brain sections to investigate gene expression • Performance of opioid receptor autoradiography and GTPgS assay on rat brain sections to study receptor activity • Image analysis of autoradiograms using the MCID program