Nadège Lagarde

Immune regulation by microRNA and Immunomonitoring of new treatment and biomaker research in Multiple Sclerosis -PBMC and extracellular vesicle (EV) isolation from patient blood -miRNA analysis fro serum or EV: qPCR, ddPCR, RNAseq -Creation and management of patient sampling and resources databases -Teaching members and non-members of the lab, EV isolation and use of related devices -Writing of experimental protocols Reporting and presenting work in progress

Development of virally vectored vaccines -In vivo mouse work, cell cultures, FACS, molecular biology, neutralisation assays, Plaque assays, ELISA -Creation and management of databases for reagents

Development of neuro-inflammation mouse model - In vivo mouse work, histology, biochemistry, cell culture, molecular biology, multiplex ELISA -Creation and management of sample databases -Writing of experimental protocols -Reporting and presenting work in progress

Role of regulatory T cells and their possible interactions with lymph node stromal cells in a mouse model of skin allograft transplantation -Mouse skin tail transplantation, stromal cell isolation (autoMACS), mulitcolo FACS, RT-PCR, ELISA, cell stimulation - Creation and management of databases for consumables, reagents and mouse colony management

Development of high-throughput/high content tool for an influenza A pseudotyped lentiviral particles seroprevalence study in populations in S-E Asia. Identification and characterisation of novel influenza virus-host protein-protein interactions. -Cell culture, siRNA, transfection, cloning, immunoblot, IFA, ELISA, -Responsible for microscopes and image analysis software teaching, -Creation of databases for consumables, reagents, BSL2 materials and alumni -Reporting work in progress

-In situ RNA hybridization on mouse embryo limb, DNA and RNA extraction, PCR, -Mouse colony management (about 15 strains) weaning, culling, genotyping, mating and pregnacy assessment, embryos collection -Database management for mouse colony -Teaching and assisting students in mice/lab work using GLP -Supervision and training of media/support staff

-Production fo primary isolate of HIV-1 -Measure of virus replication, neutralisation by HIV seropositive patient and read out by ELISA -Purification of immunoglobulins using affinity chromatography columns. -Production of Fc and Fab fragments -SDS-PAGE, coomassie blue staining -Writing and updating protocols

Molecular, genetic and histological characterization and therapeutics trials on FrdA (Friedreich Ataxia) mouse models -In vivo mouse work: IP injection, necropsy, behavioral test, mating, weaning, genotyping -Histology: cryostat, microtome, IHC, IFA -Molecular Biology: DNA, RNA extraction, RT-PCR, qPCR (Cybr green) -Cell Biology: primary cell culture (mouse muscle) -Biochemistry: Immunoblotting

Cell Biology Animal Physiology Genetics Immunology Virology