Dr. Sandrina Körner

Establishment of murine xenograft models for lymphogenic and haematogenic metastatic spread of head and neck squamous cell carcinoma and the use of potential new therapeutic agents. Tasks included cell culture, proliferation and migration assays, CRISPR-Cas9, NGS, western blot, standard molecular biology techniques, in-vivo mouse handling, small animal in-vivo imaging techniques (µCT, ultrasound, MRI), immunohistochemistry, microscopy.

The role of COX2 for the local immune modulation in MSI-H colorectal cancer (Lynch syndrome). MMR-deficient tumors in Lynch syndrome are an ideal model system for studying tumor immune surveillance and immunoediting. Tasks included immunohistochemistry, sequencing, qPCR and standard molecular biology techniques.

Institute of Pathology, Applied Tumor Biology - PD Dr. Matthias Kloor Identification and classification of the local tumour micromilieu of MSI colorectal cancers. Performance of mutation and methylation analysis, IHC stainings from FFPE tissues, entrechning a new chromogenic based double staining method in the lab. Phenotypical characterization of colorectal cancers of the MSI-H phenotype for their expression of key components of glucose metabolism and comparison of the results with MSS cancers.

Department of Medical Biochemistry and Molecular biology - PD Dr. Markus Greiner Identification of function of the Hsp40-cochaperones in the endoplasmic reticulum ERj3 and ERj6 in cultured cells. Tasks included broad spectrum of biochemical and cell biological lab techniques including Western-blotting, human cell culture, siRNA or plasmid transfection, fluorescence microscopy as well as functional cell biological approaches like cell-migration assays or real-time cell analysis.

Institute of Pathology, Rhabdomyosarcoma/Tumor Immunology - Dr. Katja Simon-Keller, Prof. Dr. Timo Gaiser Tasks included cell culture of human immortalized mammalian cells, RNA-, DNA- and protein extraction of mammalian cells, PCR, qRT-PCR, Western Blot, bacterial transformation and plasmid isolation

Coexistence of parvalbumin and GlyT2 in the auditory brainstem of mice. Thus, parvalbumin is not only a marker for GABAergic neurons in the auditory brainstem but may also indicate glycinergic neurons. Tasks included transcardiac perfusion, coronal brain slices, immunohistochemistry, fluorescence and confocal microscopy.

Heidelberg University Hospital, Institute of Pathology, Applied Tumor Biology - PD Dr. Matthias Kloor Introduction into the basic concepts of microsatellite instability analysis and glycobiology of microsatellite-unstable cancers. Performance of microsatellite instability analysis in more than 250 specimens of non-small cell lung cancer. Tasks included mononucleotide fragment length analysis and visualization using a fluorescence-based detection system.

Boveri Junior Research Group Innate Immunity, PD Dr. Adelheid Cerwenka