Annabelle Sander

My Bachelor Thesis focused on the comprehensive characterization of CK1-mediated tau phosphorylation in Alzheimer’s disease - Site-directed mutagenesis (iPCR) - Bacterial expression of recombinant protein - Purification of GST-tagged proteins via FPLC - Purification of His-tagged proteins via IMAC - Cultivation of human neuronal progenitor cells (hNPCs) - Zn2+-Phos-tag™ SDS-PAGE - Western blot analysis - Handling of radioactive material - Two-dimensional phosphopeptide analysis

Focus: “Establishment of a fluorescence-based assay detecting in vitro tau441 aggregation for the investigation of the role of CK1δ and ε in PHF formation” - Bacterial expression and purification of proteins - In vitro kinase assay for protein phosphorylation - Establishment of the PHF aggregation assay