Antu Thomas

• Cloning into expression vectors via restriction digestion/ligation or In-Fusion cloning. • Gene overexpression, protein purification, characterization and enzyme activity assays. • Random mutagenesis and screening using microtiter plates for higher activity enzymes. • Enzyme-coding genes co-expressed in strain engineered (using CRISPR/Cas9) E. coli. • Fermentation using the recombinant strain in shake flasks and 1.5 L Dasgip® parallel bioreactors.

• Operated and optimized spray drying and milling processes for producing lignin powders. • Analyzed the particle size distribution of lignin powders produced.

• Identification of test scenarios based on client requirements. • Development and documentation of test cases with step-by-step details. • Execution of test cases, identifying and reporting of the bugs. • Support development team by root cause analysis of the bugs by using the generated logs. • Have experience in running automated scripts • Peer review of test scenarios identified by the team members. • Achiever of “Lakshya Award” for outstanding performance in work in 2014.

Biocatalysis, Bioprocess, Molecular Biology, Microbiology, Cell and Tissue engineering, Separation technologies for Life Sciences, Applied Thermodynamics, Process Systems Engineering, Particle Technology, Bioresources and Biorefineries

Biochemistry, Microbiology, Molecular Biology, Enzyme Engineering and Technology, Genetic Engineering, Bioprocess Principles, Bioreactor Design and Analysis, Bioseparation Technology, Immunology, Cell Biology, Structural Biology, Chemical Reaction Engineering and Process Control