Eric Willard

Optimized and conducted a series of high throughput, thermotolerance assays to screen 5000+ enzyme variants. SDS-Page and Bradford assays. Kinetic and endpoint enzymatic reactions. Glucose Oxidase fluorescence assay. Purification using hollow fiber diafiltration.

Composed multi-fragment assemblies using type IIS restriction enzymes and three part ligation optimization. Programmed and utilized automation instruments including Tecan, Qpix, and Saffire. Qiagen based plasmid DNA isolation from bacterial colonies, restriction digestion, dephosphorylation, ligation and transformation for vector construction. PCR optimization.

Seaward sample collection using Van Veen, CREEB coring, dredging and Niskin bottle techniques. DNA extraction, PCR, Cycle Sequencing and Direct Sequencing in efforts to test for Paralytic Shellfish Poisoning.

Biochemistry, Genetics and Molecular Biology, Physics 1 and 2, Organic Chemistry 1 and 2, Analytical Chemistry, Public Speaking, Ecology, Vertebrate Zoology, Biological Oceanography, Marine Biology, Herpetology, Geology, Beginning SCUBA