Jutapak Klangprapan

Training in Mammalian cell culture (Vero cell) as host cell to produce African Swine Fever Virus (ASF) for the diagnosis development.

Project: Synthesis and Characterization of Molecular Imprinted Polymer Nanoparticles (nanoMIP) against Ferritin - Try condition for nanoMIP-ferritin synthesis - Characterization of nanoparticles - Evaluating the interaction of nanoMIP and its target - Progress report and discuss in laboratory meeting

Project: Synthesis and Characterization of Molecularly Imprinted Polymer Nanoparticles (nanoMIP) Against Porcine Circovirus Type 2 Viral Like Particles. - Synthesis of MIP nanoparticles - Characterization of MIP nanoparticles and virus - Interaction of MIP toward PCV2 by examining fluorescence quenching - Affinity binding analysis quartz crystal microbalance (QCM) - Preparing the report (presentation) in laboratory meeting - Make presentation for the conference - Publishing of scientific manuscript

Trained in ribosome display and phage display technique for selecting peptide specific-virus capsid Project: Selection of single- chain variable fragment (scFv) specific to PCV2capsid by Phage display technique - Bio-planning, ELISA screening, construct scFv in vector - scFv sequencing and CDR analysis - scFv expression and purification, SDS-PAGE, Western blot analysis - SPR, Electrochemical, protein interaction analysis - Progress report and presentation - Preparing scientific manuscript for publication

Trained in molecularly imprinted polymer and quartz crystal microbalance Project: Sensing the classical swine fever virus (CSFV) with molecularly imprinted polymer on quartz crystal microbalance - Synthesis thin-flim polymer for CSFV - Preparing quartz crystal microbalances (QCM) - Characterization of virus and MIP cavity by using Scanning Electron Microscope (SEM) - Preparing MIP-QCM sensors and measurements - Report and presentation - Preparation scientific manuscript

Project responsibilities : 1) Development of lateral flow based on aptamer for detection of organophosphate and paraquat. 2) Selection and screening of aptamer for Nosema bombycis Nageli using systematic evolution of ligands by exponential enrichment (SELEX). 3) Impedimetric immunosensor for detection of Tilapia lake virus (TILV) in Nile tilapia serum. - Participation in workshops - Teaching and organizing in laboratory - Writing project proposal for founding