Kushi Kushekhar

Bis 2015, PhD Researcher, University Medical Center Groningen
Groningen, Niederlande

Fähigkeiten und Kenntnisse

>8 years experience in Immunology
Hands on experience on measurement of immune respo
Screened the immunogenic antigens for their (anti)
Developed antigen specific T cell clones for CTL i
Established EBV transformed B cell lines (LCLs) an
Designed and developed SOPs for TGFβ1 IL10 and IFN
to measure antigen induced immune responses
Established EBV transformed B cell lines (LCLs).
Experienced in interpreting analyzing and inferrin
Thought workshops on ELISPOT and data interpretati
Communicated with international collaborators (n=4
Handled and maintained large genotyping databases
Developed patient consent forms according Medical
Excellent presentation skills in multiple internat

Werdegang

Berufserfahrung von Kushi Kushekhar

  • 5 Jahre, Apr. 2010 - März 2015

    PhD Researcher

    University Medical Center Groningen

    Association analysis of HLA alleles to susceptibility of Hodgkin lymphoma (cHL). HLA associations in cHL subgroups stratified based on age, EBV status, and HLA expression status. Screening and identification EBV latent antigenic epitopes in EBV+ cHL. T cell responses against EBV antigens in the context of HLA-A*01 and HLA-A*02. Characterization of immunogenic epitopes for potential CTL therapy. Mutation analysis of HLA-A*02 gene in tumor cells of EBV+ cHL.

  • 1 Jahr und 8 Monate, Juli 2008 - Feb. 2010

    Junior Research Fellow

    All India Institute of Medical Sciences

    - Global analysis of inflammatory cytokines in the serum of children with Rotaviral diarrhea and Chikungunya fever. - Molecular characterization (RT-PCR) and phylogenetic analysis of prevalent CHIKV strains that caused 2006 outbreak. - Flow cytometric analysis of T cells (CD8+, CD4+) and NK cells (CD56+) for their cytotoxic function by intracellular staining (ICS) of IFNg. - Evaluation of T cell IFNg response (ELISPOT) against Rotavirus membrane proteins.

  • 11 Monate, Sep. 2007 - Juli 2008

    Research Assistant

    Indian Institute of Technology (IIT) New Delhi

    Prevalence of Rotaviral diarrhea in New Delhi - Electrpherotyping (by SDS-PAGE) to identify the virulent Rotaviral strains - RT-PCR based characterization of virulent Rotaviral strains prevalent in New Delhi - Phylogenetic analysis of Rotavirus evolution

  • 6 Monate, Jan. 2007 - Juni 2007

    Masters project

    Center for clean environment technology (CCET) Bangalore University

    - Biochemical analysis of sewage water. - Isolated (salting in) and characterized metal degrading bacteria in sewage water. - Studied enzyme kinetics of immobilized (Sodium alginate method) cyanide detoxifying enzymes.

  • 2 Monate, Juli 2005 - Aug. 2005

    Internist

    Kempegowda Institute of Medical Sciences

    - Prepared various liquid and solid agar cell/tissue culture medium - Processed blood for serum/plasma separation - Isolated blood cells

  • 2 Monate, Juni 2004 - Juli 2004

    Internist

    Veterinary College, Hebbal, Bangalore

    - Cultured and maintained animal cell cultures - Performed virus neutralization assay - Maintained and sterilized of laboratory utensils

Ausbildung von Kushi Kushekhar

  • 2 Jahre und 2 Monate, Juni 2005 - Juli 2007

    Biotechnology

    Bangalore University

  • 3 Jahre und 2 Monate, Juni 2002 - Juli 2005

    Biotechnology

    Bangalore University

Sprachen

  • Englisch

    Muttersprache

  • Niederländisch

    Grundlagen

  • Hindi

    -

  • Kannada

    -

  • Telugu

    -

  • Urdu

    -

  • Sanskrit

    -

  • Tamil

    -

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