Lucia Robustini

Cloning, expression and purification of heterologue protein inbacterial vector. in vitro experiments to test enzymes activity.

Heterologues protein in bacterial vectors (cloning, expression and isolation of PDC enzyme from Ephedra spp. and A. thaliana in E. coli) Use of PDC to biocatalyze a new C-C bond, yelding a compound involved in ephedrine biosynthesis pathway. Ability in the use of analitical machinery (HPLC-MS/MS, GC-MS, FPLC) as well as biochemical techniques (PCR, SDS-page, electrophoresis, ligation and restriction test).

Biocatalyses, Biorefinery, Food Chemistry, Analitical Chemistry, Sugar Chemistry, Environmental Chemistry and Bioremedation, Plant Breeding, Microbiology, Biofuels