Ludmila Friesen

MicroBioMed Project, Research assistant •Production and purification of recombinant antigens, monoclonal antibodies (IMAC, Protein A/G Chromatography) •Characterisation of proteins and antibodies (ELISA, flow cytometry, Immunohistochemistry, Western Blot, PAGE, receptor internalisation studies, SNAP-tag technology) •Cell culture •ELISA •Immunisation/blood collection from mice, titer •DNA/RNA purification, cDNA Synthesis, PCR, cloning •Supervision of students

Master Thesis in the Plant Biotechnology Group: work on the immobilisation of Malaria vaccine candidates on the surface of CPMV empty virus like particles (eVLPs) • Production of Malaria vaccine candidates and CPMV eVLPs by transient expression in N. benthamiana • Chemical conjugation of vaccine candidates on the surface of the eVLPs • Evaluation of conjugation efficiency by agarose gel electrophoresis of proteins, gel filtration chromatography, Western blot, PAGE

Work on the development of Schmallenberg Virus Detection Assay in cooperation with The Pirbright Institute • production of viral antigens (Schmallenberg virus, Bluetongue virus (BTV)) in N. benthamiana • production of BTV eVLPs in plant: purification by density gradient ultracentrifugation, integrity investigation by TEM)

Work on the Wine Yeast Project in cooperation with Winery in Salgesh and Fraunhofer Institute in Schmallenberg • Evaluation of the native antigen recognition on the yeast cell surface in flow cytometry and fluorescence microscopy • Development of a yeast detection quick-test (evaluation of materials, geometry calculation, statistical evaluation)

•Production of HCV Antigens in E. coli (periplasmic expression) and HEK293T cells •Optimisation of Expression conditions and IMAC-purification conditions •Molecular cloning

Worke on the development of an immunological quick-test for the detection of wild yeasts in spontaneous wine fermentation • Purification of polyclonal antibodies from rabbit sera (Protein G, Äkta purifier) • Characterisation of polyclonal antibodies (PAGE, native/reducing; Western blot; titer determination) • Reduction of antibody cross-reactivity by pre-adsorption • Set-up and evaluation of a sandwich ELISA

Red Biotechnology: cell biology, immunology, protein chemistry, signal transduction, biomaterials and bioactive peptides Molecular and Industrial Biotechnology, Bioprocessing

Zoology, Botany, Anatomy and Evolutionary Morphology, Geology, Inorganic and Analytical Chemistry General education: Philosophy, Economy, Geography, Mathematics