Luis Eduardo Lucena Baeza

Article scoping, curation and quality control of the database content on somatic and germline genetic variation Assisting the IT team in SQL design and bug reporting for database QC Conference attendance and market research Project lead in the writing of ACMG-based clinical summaries on genetic variation for diagnostic laboratories Usage of NCBI biomedical resources, HGVS nomenclature as well as disease/phenotype ontologies

Mouse DNA extraction and PCR genotyping Measurement of metabolic compounds in mouse urine samples using HPLC-MS/MS Data and statistical analysis using Excel, Open Access and GraphPad Mouse colony data management using FileMaker X-ray in vivo radiography of mice Anesthesia, euthanasia and necropsy of mice Mouse ear tagging and tail snipping

Project title: epidemiology of the OXA-48 carbapenemase among Klebsiella pneumoniae isolates in Catalonia. Techniques: pulse-field gel electrophoresis, multi-locus sequence typing, microbiological cultures, MIC determination, bioinformatics.

Project title: creation of a new strategy for increasing the chances of a double recombination event using a novel vector in Staphylococcus aureus. Techniques: DNA isolation, PCR, use of cloning vectors, bacterial transformation, design of fusion proteins, fluoresence microscopy, microbiological cultures.

Determination of mineral level in flour (ICC-Standard No.104), humidity in cereals and seeds according to ISTA-methods, test- and thousand kernel weight, sample preparation.

DNA/RNA extraction and real-time qPCR quantification of microbial pathogens (HIV, Hepatits B and C) in blood and plasma samples and detection of human mutations in genes related to blood metabolism.

Courses on tissue biobanks, experimental animal models, genomics, metabolomics and proteomics, oncology and angiogenesis, bioinformatics, microscopy techniques and stem cell therapeutics

Common courses in biostatistics, experiment design, ethics in research and epidemiology, and microbiology courses focused on microbial antibiotic resistance, pathogenesis, treatments, hygiene hospital protocols, epidemiological surveillance international programmes.

Common courses in microbiology, ecology, botany, zoology, geology, biochemistry, physiology, biostatistics, mathematics, physics and chemistry and specific courses on clinical microbiology, virology, immunology, metabolism, cellular biology, experiment design and bioinformatics.