Magdiel Lim Sheng Satha

Abschluss: Msc., Universität Göttingen
Hamburg, Germany

Skills

Microsoft Excel
Team work
Word
ELISA
PowerPoint
Outlook
Plant Biochemistry
Microbiology
Molecular biology
R
Agarose Gel Electrophoresis
API tests
Artemis
Blue white screen
Biotechnology
Cell Culture
Confocal laser-scanning microscopy
DNA Extraction
DNA Sequencing
Epifluorescence microscopy
ImageJ
LDH assay
Mass spectrometry
Mauve
Molecular Cloning
MTT assay
Next generation sequencing (NGS)
Origin (Software)
PCR Primer Design
Polymerase Chain Reaction (PCR)
Photoshop
Plant breeding
Transmission Electron Microscopy (TEM)

Timeline

Professional experience for Magdiel Lim Sheng Satha

  • 6 months, Oct 2020 - Mar 2021

    Research

    Albrecht-von-Haller-Institute for Plant Sciences

    Thesis topic: Characterization of Putative Sterol Esterases in Arabidopsis thaliana • Conducted plant crossing (interbreeding) • Genetically modified plant cell through particle bombardment • Examined gene expression through RT-qPCR • Designed primer for PCR • Conducted confocal laser-scanning microscopy (CLSM) • Analyzed lipid using mass spectrometry (MS), radioisotope and thin-layer chromatography (TLC)

  • 1 month, Dec 2019 - Dec 2019

    Research Assistant

    Albrecht-von-Haller-Institute for Plant Sciences

    • Managed gene transformation tasks in plant and bacteria using gene gun and electroporation. • Utilized Epifluorescence microscopy to measure genetically modified plant cells • Analyzed data using ImageJ and Microsoft Excel

  • 3 months, Sep 2019 - Nov 2019

    Research

    Albrecht-von-Haller-Institute for Plant Sciences

    Primary Metabolites and Sterol Profiling (Metabolomics) • Extracted hydrophobic and hydrophilic metabolites • Applied Gas chromatography–mass spectrometry (GC-MS) in analyzing lipids, sugars, amino acids and alcohols • Investigated the distribution of metabolites using R and ClustVis

  • 3 months, Jun 2019 - Aug 2019

    Research

    Institute of Microbiology and Genetics

    Characterization of Nanocellulose Producing Bacteria • Measured Cell growth using Cell growth quantifier • Successfully detected nanocellulose production from bacteria • Discovered biochemical activities of novel bacteria using API 20NE and API ZYM test • Analyzed nanocellulose producing gene through bioinformatic approach (Artemis and Mauve)

  • 3 months, Nov 2018 - Jan 2019

    Research

    Institute of Microbiology and Genetics

    Analysis of Epilithic and Endolithic Bacteria • Conducted next-generation sequencing (NGS) technology • Operated transmission electron microscopy (TEM) • Carried out field work

  • 1 year and 6 months, Aug 2013 - Jan 2015

    Administrative Assistant

    Soochow University

  • 2014 - 2015

    Research

    Department of Microbiology

    Thesis topic: Cloning of the Gellan lyase gene from Alicyclobacillus acidocaldarius 49-1 • Cloned antibiotic resistant genes into bacteria • Conducted biochemical tests (Ninhydrin test, Biuret test, Lowry method) for amino acids and proteins • Conducted biochemical tests for polysaccharides (iodine test, Molish test, Bial test, Seliwanoff’s test, Benedict test) • Knowledge - LDH cytotoxicity assay and MTT assay - Enzyme-linked immunosorbent assay (ELISA) - Blue-white screen

Educational background for Magdiel Lim Sheng Satha

  • 3 years and 4 months, Oct 2017 - 2021

    Microbiology, Biochemistry and Biotechnology

    Universität Göttingen

    Plant Biochemistry, Microbiology

  • 4 years and 1 month, Sep 2011 - Sep 2015

    Microbiology

    Soochow University (Taiwan)

Languages

  • English

    Fluent

  • German

    Intermediate

  • Chinese

    First language

  • Malay

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