Muhammad Jan Brohi

Bacterial growth induction for optimal protein production Bacterial cell harvesting Cell disruption Protein purification using GE Akta purifier (FPLC) instruments using methods such as Affinity Chromatography, Anion Exchange chromatography and Size Exclusion Chromatography SDS gel analysis of the protein samples Gene isolation and amplification via PCR thermocycler Plasmid digestion and gene infusion Plasmid insertion using Heat Shock and Electroporation techniques

Specialized Media preparation for anaerobic Bacterial Growth Bacterial cell harvesting Cell disruption (bead beating and french press) Protein quantification using BSA protein quantification method Enzyme Activity Assay in COY Anaerobic Chamber Enzyme activity measurements via HPLC