Sandesh Deshpande

Sequence and activity based screening for polysaccharide degrading enzymes from extremophiles

Title: Process Development for Enzymatic Synthesis of L-glyceraldehyde-3-phosphate Tasks: - Development of an analytical method to monitor the reaction - Selection of the enzyme with optimum activity and stability - Optimization of temperature, buffer and pH and co-factor concentration - Investigation of effect of ATP/Mg2+ on activity of enzyme. - Evaluation of different ATP regeneration methods and their economic viability for industrial application. - Investigation of reaction kinetics

Title: Set-up and Characterization of a Fixed Bed Reactor for Enzymatic Polymerization - Set up of reactor system with automated sampler - Selection of optimum solvent for the reaction - Investigation of effect of residence time on conversion - Investigation of effect of residence time on degree of polymerization - Investigation of the stability of enzyme in the fixed bed reactor

- Enzyme activity assays for lipases

- Set up of a water scrubber for removal of CO2 for a 40m3 biogas plant - Microalgae culture in photobioreactors