Sanjata Joshi

Research related to SARS CoV2 and Enterovirus proteases - Recombinant protein expression in E. coli for biochemical characterization and inhibitor screening - Protein purification using chromatographic techniques (ÄKTA/FPLC, Ni-NTA affinity chromatography, ion exchange and size exclusion chromatography) - Development and execution of fluorescence-based enzymatic assays (FRET) for inhibitor screening and IC₅₀ determination - Biophysical characterization of proteins using differential scanning fluorimetry (Na

- Execution of biochemical and fluorescence-based assays for research projects - Preparation of buffers, reagents, and chromatographic materials for purification experiments - Support of crystallization experiments and analytical characterization workflows - GLP-oriented documentation of laboratory processes and adherence to SOP-based workflows - Support in troubleshooting and optimization of experimental procedures

-Screening of the microbial strain from different sample( Nose/Throat, Urine, Rectal, Variant (Wound, operational wounds,) - Isolation and Identification of microorganisms from various clinical samples ( MALDI- TOF, VITEX, Agardiffusion ) - Sample preparation to detect the parasites from stool and observation under microscope - Quality evaluation of different samples and control -Antibiotic sensitivity test -Keeping records of the test sample -PCR

-Fermentation of bacterial cellulose -Analytical comparison of different bacterial cellulose- Moisture content, Carboxylic content, TEMPO Oxidation, Green Oxidation, freeze-drying, Viscosity measurement. -Biodegradation evaluation of bacterial cellulose in soil.

International team work, helped me to improve my german language