Dr. Sebastian Hauke

- Manages projects as a lab leader - Establishes and optimizes advanced analytical methods for GMP-compliant quality control and characterization purposes of active pharmaceutical ingredients in drug substance and drug product - Drives pipeline projects while ensuring GMP-compliant documentation - Well experienced with NBE formulation, process development and quality control as well as Quality by Design (QbD) and Design of Experiment (DoE) - Expertise in statistical data evaluation and interpretation

- Established quantitative cell reporter/potency assays and advanced (bio-)analytics for small molecule screening - Communicates research results to an interdisciplinary audience in team meetings & international conferences - Delivers peer-reviewed publications - Supervised and trained an international team of bachelor to PhD scientists by designing research projects/overseeing lab experiments/thesis writing - Responsible for acquisition, performance monitoring, function of analytical instrumentation

- Implemented cellular assays & (bio-)analytical methods for the identification/quantification of small molecular factors in cell disease models/primary cells - First to demonstrate the essential role of autocrine signaling factors for β-cell activity & insulin secretion - Collaborated actively in international research efforts assuring successful and timely project completion - Published 9 peer-reviewed articles, presented scientific results as posters and talks in international conferences

- Designed and implemented a broadly applicable enzymatic / chemical protein labeling strategy, synthesizing custom-designed photoactivatable fluorophores that readily penetrate cell membranes - Monitored and characterized protein localization dynamics inside live mammalian cells using advanced confocal microscopy and quantitative (automated) image analysis - Received 2 awards in protein biochemistry for outstanding master thesis and fast / efficient studies

- Developed and synthesized new hydrazine substrate analogues for the optimization of Staphylococcus aureus sortase-mediated ligation reaction - Established enzyme assays and determined enzyme kinetics

- Identified novel proteinaceous biomarkers in tumorous tissue via mass spectrometry and bioinformatics - Used bioinformatics approaches for biomarker identification

- Designed and synthesized a fatty acid-based photoaffinity probe - Performed lipid-protein crosslinking to determine cellular lipid interactome and metabolism

- Designed, synthesized and radio-labeled bone tracing peptides after solid phase synthesis for selective drug delivery - Followed biodistribution of radio-labeled peptides in live animals using tomography and molecular imaging

- Developed a selective two-step protein labeling procedure based on enzymatic ligation & organo-metallic chemistry - Computational design of enzyme substrate specificity - Synthesized and characterized new substrate analogues

- Chemical/cell/molecular biology - Advanced cellular disease models - Immunoassays, cellular (potency) assays, target binding assays, drug potency assays in live cells - (Bio-)analytics (chromatography, mass spectrometry, spectroscopy, electrophoresis) - Metabolomics

- Protein chemistry, proteomics & bioconjugation - (Bio-)analytics & biophysics - Chemical / cell / molecular biology - Advanced microscopy - Enzymatics

- Protein chemistry, proteomics & bioconjugation - (Bio-)analytics & biophysics - Chemical / cell / molecular biology - Enzymatics