Srinivas reddy Pallerla

I. Coronaviruses Project: Coronaviruses, including SARS-CoV, MERS-CoV, and SARS-CoV-2, express a nucleocapsid (N) protein essential for viral replication, transcription, and virion assembly. A defining feature of the N protein is the phosphorylation of the serine/arginine-rich (S/R) domain located within its central disordered region. This region is highly conserved across the Coronaviridae family, suggesting a fundamental regulatory role and a promising target for broad-spectrum antiviral intervention. Ho

Research in the area of infectious disease biology, Host-Pathogen Genetics, Viral quasispecies, Next-Generation sequencing, Nanopore and Miseq, Molecular diagnostics, Pharmacogenetics

From Genome-wide association to causality: Role of novel type 2 diabetes locus Hexokinase domain containing gene 1 (HKDC1) in glucose homeostasis and metabolism • Cloned, expressed, purified and kinetics was performed on human and mouse Hkdc1 proteins. • Generated mouse Hkdc1 knockout embryonic stem cells and generated Hkdc1 knockout mice. • Identified allelic differences in regulatory activity of HKDC1 variants associated with type 2 diabetes. • CrisPR/Cas9 is currently used to study regulatory variants as

The role of the unfolded protein response in facilitating tumor evasive resistance to antiangiogenic therapy • Showed unfolded protein response induced IL-8 and VEGFA expression in renal cancer cell lines. • Studied drug resistance caused by sunitinib and taxanes in breast cancer therapy.

Roles of peroxisome proliferator-activated receptor gamma (Pparg) and its target genes pyruvate carboxylase (Pcx) and lactate dehydrogenase B (Ldhb) in mouse placental development and metabolism • Established recombineering technology in the lab. • Three targeting vectors (Ldhb, Pcx, and Estrogen receptor related beta - Cre recombinase) were generated using recombineering technology. • Generated C57BL/6 -129/sv hybrid embryonic stem cells. • Generated Esrrb-Cre mice & Ldhb conditional KO mice using genera

Role of N-deacetylase/N-sulfotransferase (Ndst) 1 and Ndst 3 gene function in mouse embryonic development and in the adult brain • Investigated the role of N-deacetylase/N-sulfotransferase (Ndst) 1 and Ndst3 gene function in mouse embryonic development and in the adult brain. • Gained very good exp

Characterization of the polyphosphate metabolism in Corynebacterium glutamicum using newly developed fluorescence microscopic and photometric methods • A method was established to monitor polyphosphate changes in vivo in C. glutamicum by epifluorescence microscopy and fluorescence photometry. • Pol

Phamaceutical sciences