Stephan Starke

Abschluss: Master of Science, Ernst-Moritz-Arndt-Universität Greifswald
Leipzig, Deutschland

Fähigkeiten und Kenntnisse

DNA Isolation
PCR Analysis
site directed mutagenesis
restriction enzyme digestion
ligation of DNA fragments
transformation in E. Coli
gelelectrophoresis/DNA and Proteins
2D SDS PAGE
Coomassie- / Silver-/ Fluorescent gelstaining
Proteinisolation
Proteinconcentration and buffer exchange
BCA/ Bradford/ Lowry proteinquant
His - Tag/ Heparin - Affinitychromatography (Äkta)
Circular Dichroism Spectroscopy
Dynamic Light Scattering Spectroscopy
Bacteria Cultivation and Physiology Assays
Cell disruption via Sonification/ Frensh Press/ Ri
sample prepartion for mass spectrometry
Gas Chromatography

Werdegang

Berufserfahrung von Stephan Starke

  • 4 Monate, Feb. 2018 - Mai 2018

    Graduate Student Research Assistent

    Helmholtz-Zentrum für Umweltforschung

    Bacterial degradation of industrial Polyurethane samples.

  • 10 Monate, Aug. 2017 - Mai 2018

    student research assistent

    Microbiology department, University of Leipzig

    Enzymatic synthesis, Isolation and Separation of large ring cyclodextrins.

  • 1 Jahr und 5 Monate, Okt. 2016 - Feb. 2018

    Masterthesis

    Helmoltz Centre of Environmental Research , Dep. of Env. Biotechnology

    Master Thesis in EU - Project " From Plastic Waste to Plastic Value using Pseudomonas putida synthetic Biology". Physiology Assays wih Pseudomonas putida

  • 5 Monate, Mai 2016 - Sep. 2016

    student research assistent

    Transfusion medicine, university hospital greifswald

    Preparation of gold coated nanorods with immobilized proteins to image blood platelet activation under a fluorescent microscope

  • 2 Monate, Juni 2016 - Juli 2016

    Summer Course "Arctic Microbiology"

    University Centre of Svalbard

    Arctic environmental microbiology. Laboratory work, lectures and fieldtrips included in the course. Final grade was gained out of an oral exam and a laboratory report. Habitats investigated: soil, water (different depths), snow, glacier Ice, glacier meltwater, acid mine drainage

  • 8 Monate, Sep. 2015 - Apr. 2016

    student research assistent

    Department of Biochemistry, University of Greifswald

    Isolation of iminreduktases for biokatalysis assays out of E. coli via HisTag affinitychromatography. Experience in using "Äkta prime and Äkta explorer" gained

  • 6 Monate, März 2015 - Aug. 2015

    Research Assistent

    University of Torino, Department of Biochemistry

    Cultivation, Protein Isolation and 2D SDS - PAGE on Proteome of probiotic strain (industrial interest) Lactobacillus helveticus.

  • 1 Jahr und 3 Monate, Okt. 2013 - Dez. 2014

    students research assistent

    ZIK HIKE Greifswald

    expression and isolation of recombinant platelet factor 4 and characterization of PF4 - heparin complexes. biggest work experience so far. Methods ranged from building the PF4 Gene into E. coli via molecular biology over expression and isolation/ protein folding/ concentration/ separation and spectroscopic characterization of PF4 - heparin complexes.

  • 1 Monat, Jan. 2014 - Jan. 2014

    students research assistent

    Ernst Moritz Arndt Universität Greifswald

    Isolation of P. aeruginosa outer membrane proteins

  • 2 Monate, März 2013 - Apr. 2013

    Laboratory Assistant

    Biotechnology-Biomedicin Centre in Leipzig

    Processing and characterization of human skin biopsy samples from patients with dermal burns. Experiments done within a clinical study "A multicenter study on regenerative effects of low-dose erythropoietin, LDE, in burn and scald injuries". Prof. Dr. med. Augustinus Bader Address: Deutscher Platz 5, Room 1.206, 04103 Leipzig Phone: 0341 97- 31 351 Fax: 0341 97- 31 359 E-Mail: augustinus.bader@bbz.uni-leipzig.de

Ausbildung von Stephan Starke

  • 3 Jahre und 11 Monate, Apr. 2014 - Feb. 2018

    Molecular Biology and Physiology

    Ernst-Moritz-Arndt-Universität Greifswald

    Environmental Microbiology Biotechnology Biochemistry

  • 3 Jahre und 6 Monate, Okt. 2010 - März 2014

    Biology

    Ernst-Moritz-Arndt-Universität Greifswald

    Microbiology

Sprachen

  • Deutsch

    Muttersprache

  • Englisch

    Fließend

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