Alba Rúbies Rovira

- Semen analysis (macroscopic and microscopic) and preparation (simple washing, swim-up, density gradient) - Oocyte and embryo vitrification/thawing - Embryo evaluation using time-lapse system and embryo grading - Media and dish preparation for oocyte and embryo - Practice in oocyte handling, decumulation and identification of mature oocytes - Initial training in ICSI - Observation of embryo transfer - Attendance at gynaecological and biological consultations

Training at Eugin Balmes IVF laboratory included oocyte and embryo vitrification with Kitazato and Vitrolife protocols, sperm preparation and semen analysis with CASA system. Other sessions covered embryo morphology evaluation, handling transfer procedures and oocyte retrieval techniques. Additionally, hands-on sessions at PCBarcelona focused on sperm DNA extraction techniques.

Temporary contract. Performed physicochemical analyses (pH, dry extract, density, acid reserve), supported formulation and formula modifications, assisted in implementing R&D methodologies to verify new formula effectiveness, and conducted stability and compatibility tests.

Bernhagen’s lab - Vascular Biology Bachelor's Final Thesis: The role of MIF-2 in hepatic lipogenesis and steatosis. Supervisor: Jürgen Bernhagen - PI My work focuses on studying the role of MIF-2 in hepatic lipogenesis and steatosis, exploring its impact on PI3K/AKT signaling pathways and key gene interactions, aiming to identify potential therapeutic targets for cardiovascular and liver diseases.

Performed sample classification, DNA/RNA extraction, pathogen inactivation, and PCR analysis as part of routine laboratory operations and diagnostic processes.

Handled tasks related to autoimmunity, immunodeficiencies, human leukocyte antigen (HLA) analysis, immunochemistry techniques, and allergy diagnostics in laboratory operations.