Dr. Carla Pawlowski

Experience: Protein extraction and quantification Isoelectric-focalization; two-dimensional electrophoresis; SDS-PAGE DNA extraction of genetically modified (GM) bean; primers/probes design for detection of GM bean in food

Experience: RNA isolation; denaturing gel analysis; quantification; fluorescent labeling Microarray hybridization and analysis Multivariate statistics (PCA, SIMCA)

Experience: RNA and DNA: extraction, gel analysis, quantification and purification Primer design, digestion, ligation, cloning, transformation Reverse transcription, PCR, qRT-PCR Seed germination, plant growth media formulation

Experience: Business management, budget planning, selection and training of staff Costumer service organization, hospitality coordination Management of food & beverage, events organization, serving foods and drinks Constant innovative ideas for costumer satisfaction

Experience: Technical and operational system control during beer manufacturing Data analysis focusing on product improvement and quality assurance, as well as costs and processing time reduction

Application of transcriptomics and proteomics techniques as complementary tools for food assessment by multivariate analysis

Effect of nitric oxide on the expression of genes involved on flavonoid synthesis