Danuta Zofia Korona-Burgy

Establishment of a cellular screening system for identification of miRNAs affecting recognition of melanoma cells by tumor antigen-specific T cells Techniques and Methods: T cell Isolation, Cell Culture, ELISPOT, siRNA Transfection, Flow Cytometry, Protein Isolation, Western Blot.

Analysis of the role of specific microRNAs in CD8+ T cell differentiation. Techniques and Methods: Flow Cytometry, Production of Retroviruses, T cell isolation, Transduction of murine T cells, Animal Experimentation, Analysis of miRNA Expression.

Characterisation of Evi-depleted endothelial cells. Techniques and Methods: Cell Culture, siRNA-mediated Gene Silencing, Fluorescent Microscopy, Protein Isolation, Western Blot, qRT-PCR.

Investigation of interacting partners in SOCE mechanism and possible connections to Alzheimer’s disease. Techniques and Methods: Cell Culture, Yeast-2-Hybrid Assay, Fluorescent Microscopy, Plasmid Transfection, Protein Isolation, Western Blot.

Identification and functional characterization of novel organ-specific Wnt target genes. Techniques and Methods: Experimentation on Frog Embryos, In vitro Transcription, mRNA Injection, Embryo Imaging, qRT-PCR.

Field of specialisation: Immunology, Tissue-resident macrophages of liver and spleen. Techniques and Methods: Flow Cytometry, Fluorescence-Activated Cell Sorting, Gene Expression Profiling. Courses: FELASA B, FACS, qRT-PCR, Chipster, Animal Experimentation, Thesis Writing, Job Application.

Lectures: Frontiers in Bioscience, Molecular Mechanisms of Cancer, Tumor Immunology, Virology and Cancer; Practicals: Molecular, Genetic and Cellular Approached in Cancer Research, Advanced Methods in Cell Biology, Components and Mechanisms of Signal Transduction, Immunological Methods; Internships

Biotechnology in Animal Production and Healthcare Selected courses: Genetics, Genetic Engineering, Molecular Biology, Animal Physiology, Biotechnology in Plant and Animal Production, Biotechnology Process Engineering, Biotechnology in Animal Reproduction