Dr. Dharmendra Pandey

• Qualification and Validation of scientific instruments: IQ, OQ, PQ, and MQ • Microbial load testing for environment, water, and air • Creation and reviewing of technical reports, SOPs, Deviations/CAPA-reports and trainings modules • Temperature calibration and life-cycle managements of instruments • Contact-person in DACH region for technical support to customers • Highly skilled in Lab automation, Image analysis, LIMS and Data integrity • Management of customers’ account on Salesforce

Certificate for GMP-Expert in Pharmaceuticals (TÜV ceritified): Personalmanagement, Qualitätskontrolle, Qualifizierung und Validierung, Beschaffung und Logistik (GDP) Certificate for Internal Auditor (Habmann certified): Organisation und Führung des PQS, Dokumentationsmanagement, Audits und Inspektionen, Abweichungs-/Capa Management und Qualitätsverbesserung

Theoretical and practical knowledge of: • Laws (AMG) and stakeholders in pharmaceutical business • Structure of health care systems, planning and implementation of clinical research (Monitoring, GCP, ICH) • Regulatory and medical affairs, drug safety, quality management, medical communication, and pharmacovigilance • Marketing and sales in pharmaceutical business, and creation and presentation of marketing projects • Project management and basics of Business Administration

I worked in the interface of innate immunity, virology, oncology, and immunotherapy of tumors. Also explored my previous expertise in hematology to study pattern recognition receptor in megakaryocytes and platelets biology. Some projects were: - Role of TRIM9 in antiviral immunity and Hepatitis-C infections - Study of common variable immune deficiencies (CVID); Hyper-IgM syndrome with lymphoproliferative disorder and Hyper-IgE syndrome - CAR T-cell therapy against solid tumors

I generated and analyzed knock-out/-in mice carrying different mutants of β1 integrins. I tried to define the outer-clasp of the β1 integrin transmembrane domain which plays an essential role in the regulation of β1 integrins activation. Some key expertise were mouse genetics, immune-techniques, and intracellular export, stability and recycling of cell surface receptors. I explored my previous knowledge in platelet- and microparticles-biology to collaborate with other working groups and institutions.

I unravel the signaling molecules linking the PAR-/LPA-receptors to cofilin-dephosphorylation and granular secretion in activated platelets. We coined the Rac1-PAK-Cofilin-phosphatase signaling pathway responsible for this process and suggested to target coilin phosphatase for prevention of platelets pathological role during thrombosis and atherosclerosis. Some experimental skills applied were western blotting, platelet biology, flow cytometry, antibody screening and drugs/inhibitors dose-response testing.

I worked on signaling involved in the progression and prevention of thrombosis and atherosclerosis during my Ph.D. It involved deciphering the regulation of cofilin-phospho cycle and its role during platelet and endothelial cells activation by physiological as well as pathological agonists. We hypothesize that enzymes regulating the cofilin phospho-cycle in platelets and endothelial cells could be potential targets for the development of anti-thrombotic drugs and cardiovascular diseases.

Platelet Biology, Signal Transduction, Cardiovascular Biology, Immunology, Hematology, Atherosclerosis and Thrombosis

Molecular Biology, Biomolecular Spectroscopy, Analytical Biochemistry, Molecular Biophysics, Cell Biology, Metabolism & Bioenergetics, Molecular Enzymology, Genetic Engineering, Molecular Immunology, Bioinformatics, Industrial Microbiology and Environmental Studies

Reaction mechanism in Organic Chemistry, Physical Methods in Inorganic Chemistry, Synthetic Organic Chemistry and Spectroscopy , Kinetics and Thermodynamics in Chemistry, Stereochemistry of Organic Reactions and Bioinorganic Chemistry

Chemistry, Microbiology, Botany, Zoology, Biotechnology, Molecular Biology and Biochemistry