Jessica caroline

·       Review HPLC /GC data for GMP compliance and data integrity ·       Verify integration parameters, system suitability, and analytical results ·       Review raw data, analytical reports, CoA , audit trials and processing methods in empower and LIMS ·       Handle OOS and OOT investigations and documentation review ·       Work with Empower, eLIMS and QA team for batch release ·       Support internal and external audits by providing compliant documentation Software: Empower, LIMS, ETQ

Senior Chemical laboratory Technician, Eurofins Biopharma product testing, Hamburg. ·       Extensive expertise in method development, qualification, validation, verification, transfer of analytical methods ·       Physical and chemical quality control of pharmaceuticals and semi-finished products, GMP-compliant documentation. Wet chemical and instrumental analytical methods according to the American (USP), European (EP), Japanese (JP) and Chinese (ChP) pharmacopoeias. ·       Skilled in chemical analysis,

TITLE: Functional and Taxonomic Analysis of Biomass which is anaerobically digested using pure glycerol. • Protein Extraction and purification from Biomass. • Peptide mixture separation using UltiMate 3000 RSLCnano ultra HPLC system • Analysis using timsTOF Pro tandem mass spectrometer • Meta Proteome Analyzer (version 3.1) was used to edit the protein data • NMDS plotting • MS data were compared with the UniProtKB/SwissProt database • Sorted to metaproteins using KEGGs Orthology. • Data-dependent and

Title: Optimization of the experimental and bioinformatic workflow of metaproteomic sample analysis. • Protein extraction and purification using SDS PAGE and sample preparation for MS analysis. • Data dependent and data independent quantification of proteins using various data bases. • Preparation of protein libraries from both DDA and DIA and comparison for better library.

Department of Molecular Diagnostics. Sample preparation and processing, extraction of bacterial, viral and human DNA and RNA (manual, semi-automatic and fully automatic) from patient samples with subsequent qRT-PCR set-up. Data evaluation and technical validation of findings. Qualitative and quantitative detection of pathogens.

TITLE: Anaerobic co-digestion of sewage sludge using pure glycerol and metaproteomic analysis of the biomass using LCMS study and metaproteome analyzer. Techniques: • Batch experiments using AMPTS • Fed-batch Experiments using bioreactors • Detailed investigation of feeding event • Physico chemical analysis • DNA extraction and 16s rRNA amplicon sequencing • Protein Extraction and metaproteomics.

Protein production and purification from various environmental samples using lab-scale bioreactors, biofilters, and USAB reactors. • Data-dependent and independent quantification of proteins from the LCMS data. • Isolation and maintenance of bacterial (E coli, Pseudomonas fluorescens), viral (AHA phages), mammalian (Vero cell lines) cell cultures. • Fluorescent labeling of AHA phages by click chemistry, specific adsorption of fluorescent lambda phages to host cells, fluorescence microscopy, flow cytometry

·       Method Development, Qualification, Validation, Verification, Transfer and application of analytical techniques, particularly in the context of biopharmaceuticals like monoclonal antibodies (mAbs) and protein therapeutics. ·       In-depth In-Process Analysis and Stability Studies. ·       Analytical Similarity Assessments and QTPP (Quality Target Product Profile) Assessments. ·       Routine Analytical Activities like Protein Concentration Estimation (UV spectrophotometry), Purity Analysis (CE-SDS),

Labwork •FusionPCR: PCR Amplification, Ligation, Transformation, Sanger sequencing, ColonyPCR, Cellcloning. •Microsatelliteanalysis: Capillary electrophoresis, DNA purification, Amplification of Genomic DNA, FragmentAnalysis. •CRISPRCas9 (Genome editing method): Transformation of Ecoli-pKW5 cel

Bachelor Thesis: Bioremediation of Industrial Effluents using Aspergillus flavus. Fungal culturing, Fenton treatment, Biological treatment, UV Vis spectrophotometry.