Gowthaman NR

Perform end-to-end Sanger sequencing workflows, including DNA extraction, PCR product purification, BigDye Terminator sequencing, and capillary electrophoresis. Operate ABI 3730/3730xl Genetic Analyzers and perform chromatogram QC, sequence data analysis, and troubleshooting. Handle high-throughput 96-well sample processing and microbial identification. Maintain laboratory documentation and follow established SOPs and quality requirements, expand skills Next Generation sequencing, library preparation

Responsible for Sanger sequencing and data analysis, genomic DNA and RNA extraction, plasmid isolation, DNA quality assessment, and gel purification. Operated ABI 3730/3730xl DNA Analyzers and managed sequencing file creation, plate linking, data processing, and export.

Performed nucleic acid extraction, avian DNA sexing using blood, feather, and eggshell membrane samples, and PCR-based disease screening for conditions such as Psittacine Beak and Feather Disease (PBFD). Conducted PCR and gel electrophoresis, provided technical training, interpreted experimental results, and maintained QC documentation.

Als Molecular Biology Trainee bei Plus Care International Pvt. Ltd. sammelte ich praktische Erfahrungen in der molekularen Diagnostik. Zu meinen Aufgaben gehörten RT-PCR-Tests zum Nachweis von SARS-CoV-2, RNA-Extraktion und Probenvorbereitung. Zudem wurde ich im Umgang mit den diagnostischen Plattformen GeneChecker und Accula geschult. Während der COVID-19-Pandemie unterstützte ich eine schnelle und zuverlässige Diagnostik.