Itisha Yadav

- Master’s thesis conducted at the Institute of Virology, Technical University of Munich - Conducted in vitro research in virology and immunology to study virus–host and immune cell interactions - Designed and performed cell-based assays using mammalian cell culture techniques - Performed molecular cloning, isolation of human peripheral blood mononuclear cells (PBMCs), and viral transduction as part of experimental workflows - Applied confocal microscopy and immunofluorescence staining for high-resolution

- Supported ongoing virology research projects through routine laboratory work - Maintained mammalian cell cultures and prepared samples for experiments - Performed and supported molecular cloning experiments, including plasmid preparation and validation - Conducted and supported transient transfection experiments - Assisted with immunofluorescence staining and microscopy sample preparation - Supported data collection and basic data analysis - Followed laboratory safety guidelines and standard operating pro

- Designed and prepared nanoparticle formulations for nucleic acid delivery into mammalian cells - Characterized and optimized nanoparticles for cellular uptake and delivery efficiency - Performed cell culture experiments to evaluate nanoparticle-mediated nucleic acid delivery

- Conducted DNA extraction and phylogenetic analysis of Gamochaeta/Gnaphalium plant samples from the Azores - Analyzed species distribution patterns across the archipelago to investigate potential evolutionary origins