Jakob Holzapfel

R&D - Regulatory Information Management (RIM) - Vault RIM

AG Freund - Protein Biochemistry Functional characterization of non-classical MHC II proteins: cloning and mutagenesis of DNA constructs, protein expression in insect cells via a baculovirus expression system, purification via various chromatography methods (affinity, size exclusion, ion exchange), biochemical investigations (ELISA, fluorescence polarization, thermal stability assays, SDS-PAGE, Western blot, etc)

Masterarbeit in der AG Freund - Protein Biochemistry: "Functional characterization of natural variants of human leukocyte antigen: HLA-DM and -DO": Hauptaugenmerk war der Einfluss des Polymorphimus auf das nicht-klassische MHCII-Protein HLA-DM und dessen katalytische Aktivität. Methoden: Klonierung, Baculovirus-Expressionsystem in SF9 Insektenzellen, Proteinaufreinigung (Affinitätschromatographie, Größenauschlusschromatographie), Fluoreszenz-Polarisation, SDS-PAGE, Western Blot, Thermal Shift Assay

Forschungsprojekt in der AG Dürr (Mucosal Immunity) - Untersuchungen zu angeborenen lymphoiden Zellen der Klasse 2 (ILC2s) mithilfe von Durchflusszytometrie und biochemischen Assays wie ELISA

Research project in the AG Freund - Protein Biochemistry Main focus was on cloning, protein expression and purification, cell culture with human cells and ELISA assays.

AG Freund - Protein Biochemistry (Cloning, Protein expression, cell culture, ELISA)