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Jakob Holzapfel

ist offen für Projekte. 🔎

Angestellt, Associate Consultant, Veeva Systems
Bis 2023, Biochemie, Freie Universität Berlin
Berlin, Germany

Skills

Zellkultur
Proteinbiochemie
FACS
ELISA
Fluoreszenzmikroskopie
Biochemie und Molekularbiologie
Microsoft Word
Microsoft Excel
Immunologie
IT-Consulting
IT-Projektmanagement
Regulatory Affairs
Veeva Vault
Medizin & Life Sciences
Implementation
Software as a Service (SaaS)
Workshop facilitation
Beratung
Biochemie
Genexpression

Timeline

Professional experience for Jakob Holzapfel

  • Current 1 year and 8 months, since Oct 2023

    Associate Consultant

    Veeva Systems

    R&D - Regulatory Information Management (RIM) - Vault RIM

  • 2 years, Oct 2021 - Sep 2023

    Studentische Hilfskraft

    Freie Universität Berlin

    AG Freund - Protein Biochemistry Functional characterization of non-classical MHC II proteins: cloning and mutagenesis of DNA constructs, protein expression in insect cells via a baculovirus expression system, purification via various chromatography methods (affinity, size exclusion, ion exchange), biochemical investigations (ELISA, fluorescence polarization, thermal stability assays, SDS-PAGE, Western blot, etc)

  • 10 months, Jul 2022 - Apr 2023

    Master Thesis

    Freie Universität Berlin

    Masterarbeit in der AG Freund - Protein Biochemistry: "Functional characterization of natural variants of human leukocyte antigen: HLA-DM and -DO": Hauptaugenmerk war der Einfluss des Polymorphimus auf das nicht-klassische MHCII-Protein HLA-DM und dessen katalytische Aktivität. Methoden: Klonierung, Baculovirus-Expressionsystem in SF9 Insektenzellen, Proteinaufreinigung (Affinitätschromatographie, Größenauschlusschromatographie), Fluoreszenz-Polarisation, SDS-PAGE, Western Blot, Thermal Shift Assay

  • 4 months, Feb 2022 - May 2022

    Research Project

    Charité - Universitätsmedizin Berlin

    Forschungsprojekt in der AG Dürr (Mucosal Immunity) - Untersuchungen zu angeborenen lymphoiden Zellen der Klasse 2 (ILC2s) mithilfe von Durchflusszytometrie und biochemischen Assays wie ELISA

  • 5 months, Jun 2021 - Oct 2021

    Research Project

    Freie Universität Berlin

    Research project in the AG Freund - Protein Biochemistry Main focus was on cloning, protein expression and purification, cell culture with human cells and ELISA assays.

  • 5 months, Feb 2021 - Jun 2021

    Research Project

    Freie Universität Berlin

    AG Freund - Protein Biochemistry (Cloning, Protein expression, cell culture, ELISA)

Educational background for Jakob Holzapfel

  • 4 years and 6 months, Apr 2019 - Sep 2023

    Biochemie

    Freie Universität Berlin

  • 2 years, Apr 2017 - Mar 2019

    Biochemie

    Freie Universität Berlin

Languages

  • German

    First language

  • English

    Fluent

  • Spanish

    Basic

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