Dr. Lorenz Eing

Topic: Deciphering the role of Aurora-A in R-loop homeostasis in high-risk neuroblastoma • Discovered a new complex protein network responsible for R-loop homeostasis using biochemical and Next Generation Sequencing techniques like eCLIP and DRIPc • Showed synergistic effects targeting Aurora-A and other targets • Demonstrated new RNA-dependent function of Aurora-A using gradient-sedimentation and isotope-based kinase assays with recombinant proteins • Co-authored one publication in medicinal chemistry

Topic: Targeting the Aurora-A/MYCN axis in high-risk neuroblastoma using targeted protein degradation • Established an optimized HTS-compatible in vitro cascade for PROTAC characterization. • Identified lead compounds for pre-clinical studies by screening 400+ Aurora-A targeting PROTACs. • Secured strategic funding via a successful GO-Bio (BMFTR, 600k Euro) grant to transition research toward commercial viability; performed market analysis and participated in Freedom to Operate (FTO) assessments.

Topic: NFAT5 controls the integrity of epidermis • Supported ex vivo work by mouse handling and purification of T cells from spleens and lymph nodes using positive bead-selection kits for different CD-markers • Purified primary keratinocytes from murine embryonic skin and tail skin by enzymatic digestion protocols followed by cell culture sample preparation for qPCR and immunoblotting • Organized lab inventory and orders for the group

Topic: Investigation of mTOR/PI3K and HSP90 inhibition in combination with irradiation of GBM cells • Investigated synergistic effects of mTOR/PI3K/DNAPK and HSP90 inhibition combined with irradiation in GBM cells on cancer cell survival using colony formation assays and collective- and single-cell migration assays • Showed DNA-damage and influence on signalling pathways regulating cell survival and cell migration using immunoblotting and flow cytometry analysis