Markus Held

Institute of Applied Microbiology Project: Establishing a continuous Fermentation of a recombinant Pseudomonas strain for the production of 3-(3-hydroxyalkanoyloxy) alkanoic acids (HAAs) - Cultivation of Pseudomonas taiwanensis in "Eppendorf DASBox" and "Eppendorf BioFlo 120" bioreactors - In situ HAA removal via foam fractionation - Analytical measurements (HPLC, Photometer, etc.)

Institute of Biotechnology Project: High throughput measurement of active monooxygenase concentration - cultivation of E. coli - Analysis of protein activity by using 96-well MTP based assay

Fluid Process Engineering, AVT-Aachener Verfahrenstechnik Project: "Model in situ extraction of microbiological produced itaconic acid" - reactive extraction of itaconic acid in a liquid/liquid phase system - pH shift cristalization of itaconic acid

Biochemical Engineering, AVT-Aachener Verfahrenstechnik Project: "Validiation of a measuring station for the well-resolved monitoring of cultivations in microtiter plates by simultaneous use of the Biolector and µRAMOS technology" - Cultivation of E. coli and H. polymorpha under growth-limiting conditions in MTP format - Online monitoring of growth, oxygen uptake rate and fluorescence

Project: Development and establishment of a process to coat texturised silicone implants with recombinant spider silk proteins - Building and testing of a coating device - Characterising of the quality of the coating via laser scanning microscopy - Testing the durability of the coating

Project: "Development of a scaled-up device for the production of microbeads made of recombinant spider silk proteins" - Maintained a fed batch bioreactor - Contributed to the R&D division as well as to the up- and downstream processing divisions

- CNC turning and milling - Manufactoring of prototypes for the R&D division