Mauricio Troncoso

Title: “NxtGenWood: converting wood-based phenols to value-added products” • Developed microbial fermentation processes in flask and bioreactor systems (batch/continuous) for biopolymer production from wood-derived substrates • Performed strain engineering of Pseudomonas putida using CRISPR/Cas9 genome editing • Applied analytical techniques including BioLector, HPLC, GC, PCR, RT-qPCR, agarose gels • Worked according to SOP-based procedures maintaining structured lab documentation • Designed and executed ex

Prepared and evaluated research project documentation for innovation funding applications and coordinated communication with industrial clients.

Title: “β-hydroxyaspartate cycle analysis in Pseudomonas putida” • Molecular cloning methods (electroporation, heat-shock, plasmid extraction) • Transformation of various bacterial species including Pseudomonas putida, growth assays on engineered bacterial strains • Spectrophotometric- based enzymatic assays

Title: “Investigation of light-stimulated growth of fungi” • Cultivation of Aspergillus nidulans under different medium compositions and light conditions • Use of biochemical and imaging methods to quantify growth, including adsorption spectroscopy, HPLC analysis of ergosterol and exogenous pigments and statistical methods

Title: “Investigation of interactions of the E1 alpha-helix of Tim44 with environmental proteins” • Techniques used included molecular cloning methods, such as site-directed mutagenesis, plasmid ligation and transformation in yeast • TIM Protein-protein crosslinking via UV and further analysis via protein chemical methods such as SDS-PAGE and Western-blot • Isolation of mitochondria and in-organello crosslinking of TIM proteins

1 year of master studies at the Technical University of Munich (of a two year program).