Md Imran Hossain

Topic: The antiviral potential of natural products in the treatment of infections caused by Influenza Viruses. Technical Skills Utilized: Mammalian Cell culture, fermentation, extraction, cytotoxic assay, enzyme assay, viral assay, immobilized trypsin purification, HPLC, mass spectrometry, bioinformatic analysis. Research Findings: New trypsin inhibitors from actinobacterial strains, antiviral efficiency of discovered inhibitors.

Topic: Analysis of Extracellular Nucleases in the Culture Media of V. longisporum: dsRNA degradation activity and Biochemical Approaches Technical Skills Utilized: Fungal Cell culture, FPLC, Desalting, IEC, Agarose Gel electrophoresis, SDSPAGE, In-solution trypsin digestion, Protein quantification, Mass spectrometry, Bioinformatic analysis Findings: Peptide sequence matched with putative nucleases, V. longisporum secrets nucleases directing the fate of RNAi as a tool to control fungal virulence.

Topic: Process and strain optimization towards the isolation of Darobactin and derivatives. Methods: Culture of photorhabdus isolates, optimization of fermentation conditions, data evaluation of purified peptide sequences. Technical Skills Utilized: Bacterial cell culture, C18 Protein purification, HPLC LC-MS, comprehensive bioinformatic tools. Research findings: Optimized fermentation conditions increased the yield of pure substance.

Topic: Discovering novel compounds from pharmaceutically important mangrove plants. Methods: Plant material extraction, designing and implementing of bioassays for discovering bioactive compounds. Technical Skills Utilized: Spectrophotometry, antimicrobial assay, total antioxidant capacity (TAC) measurement, cytotoxic assay Research Findings: Bioactivities of several plant extracts were recorded.