Robert Engel

Establishment of a purification process for autoantigenic proteins from human granulocytes in the context of a master thesis. Duties included: Isolation of human neutrophil granulocytes from whole blood samples, using an upscalable process. Testing of different purification methods like affinity, ion-exchange, size-exclusion and hydrophobic interaction chromatography, including column and media preparation. Checking of protein quantity and quality using SDS-PAGE, western blotting and quantitative ELISAs.

Student research assistant for production of cellbased immunoassays. Duties: passaging of Hep-2 cells and crithidia, preparation of object slides, cell seeding, fluorescence staining for quality assurance

Student research assistant at the working group for behavioural physiology of PD Dr. Ricarda Scheiner. Investigation of molecular mechanisms of honey bee behaviour. Duties: q-PCR analysis, SDS-PAGE and quantitative western blot, isolation and preparation of brain and abdominal tissue, preparation of tissue slices, quantification of sensoric sensitivity for gustatory stimuli, olfactory conditioning experiments, take care of honey bees (change combs, treatment of varroa, selective collection)

Independent supervision of an experiment for the internship "animal physiology". Duties: preparation, execution and assessment of oral exams, thematic introduction, reply to questions

Molecular biology and physiology