Robert Engel

Student, Biochemistry and molecular biology, University of Potsdam
Berlin, Deutschland

Fähigkeiten und Kenntnisse

ELISA
protein purification
chromatography (LPLC)
SDS-PAGE
Western-Blot
DNA and RNA extraction
cDNA synthesis
q-PCR
sterile working
cell culture (Hep-2 and crithidia)
production of cellbased immunoassays
tissue slicing
fluorescence microscopy
confocal microscopy

Werdegang

Berufserfahrung von Robert Engel

  • 1 Jahr und 3 Monate, Feb. 2015 - Apr. 2016

    Student Research Assistant

    Generic Assays GmbH

    Establishment of a purification process for autoantigenic proteins from human granulocytes in the context of a master thesis. Duties included: Isolation of human neutrophil granulocytes from whole blood samples, using an upscalable process. Testing of different purification methods like affinity, ion-exchange, size-exclusion and hydrophobic interaction chromatography, including column and media preparation. Checking of protein quantity and quality using SDS-PAGE, western blotting and quantitative ELISAs.

  • 5 Monate, Okt. 2014 - Feb. 2015

    Student Research Assistant

    Generic Assays GmbH

    Student research assistant for production of cellbased immunoassays. Duties: passaging of Hep-2 cells and crithidia, preparation of object slides, cell seeding, fluorescence staining for quality assurance

  • 1 Jahr und 4 Monate, Apr. 2013 - Juli 2014

    Student Research Assistant

    University of Potsdam

    Student research assistant at the working group for behavioural physiology of PD Dr. Ricarda Scheiner. Investigation of molecular mechanisms of honey bee behaviour. Duties: q-PCR analysis, SDS-PAGE and quantitative western blot, isolation and preparation of brain and abdominal tissue, preparation of tissue slices, quantification of sensoric sensitivity for gustatory stimuli, olfactory conditioning experiments, take care of honey bees (change combs, treatment of varroa, selective collection)

  • 3 Monate, Apr. 2014 - Juni 2014

    Student Research Assistant

    University of Potsdam

    Independent supervision of an experiment for the internship "animal physiology". Duties: preparation, execution and assessment of oral exams, thematic introduction, reply to questions

Ausbildung von Robert Engel

  • Bis heute 11 Jahre und 10 Monate, seit Okt. 2013

    Biochemistry and molecular biology

    University of Potsdam

  • 3 Jahre, Okt. 2010 - Sep. 2013

    life sciences

    University of Potsdam

    Molecular biology and physiology

Sprachen

  • Deutsch

    Muttersprache

  • Englisch

    Fließend

  • Russisch

    Grundlagen

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