Simon Fischer

Wissenschaftler in der biopharmazeutischen Entwicklung

Mammalian cell line development

Doctoral student at the international PhD programme Molecular Medicine

- Enhancement of biopharmaceutical production cell systems using microRNAs. - Functional high-throughput miRNA screening in recombinant CHO cells. - Cloning of mammlian expression vectors for microRNA overexpression / inhibition. - Establishment of stable miRNA overexpressing production cells. - Functional miRNA analysis in various cancer cell lines. - Development of innovative strategies to treat in vitro tumor models. - Discovery of novel apoptosis-inducing miRNAs for cancer therapy.

- microRNA research in various mammalian cell types - Comprehensive nucleic acids and protein analysis - Establishment of a microRNA target identification assay.

- Evaluation of the novel human CAP-T cell system for transient protein expression. - Process development for high-yielding transient gene expression. - Optimization of non-viral DNA transfection technologies. - Production of recombinant antigens (including fuction proteins) and antibodies using stat-of-the-art mammalian expression hosts (CAP/CAP-T, HEK293E, HKB11, CHO-EBNA). - Scale-up of transient protein production processes up to 3L culture volume.

- Establishment of recombinant mammalian cell lines (e.g. CHO, HEK293) for functional in vitro pharmacological studies. - Development of a novel non-invasive method for studying GPCR activity based on impedance measurement. - Biochemical In vitro assay development.

Applied microRNA research in mammlian cells.

Next-generation cell engineering of biopharmaceutical production systems using microRNAs.