Tamilarasan Selvaraj

Independent operations management, data administration, and structured team handovers. Provided professional overnight guest relations, managing check-ins/check-outs, reservations, and billing. Conducted security sweeps and ensured smooth, safe nighttime operations. Managed cross-functional communication and organized structured shift handovers to the day team.

Coordinated shipping lines (final packaging) and acted as a key point of contact for dispatching/scheduling. Managed problem-solving, quality control, and technical support using Jira and internal tools. Led communication-based team coordination and handled ticket creation for specialized departments.

- Designed and developed vaccines using intracellular yeast expression systems - Optimized single-step VLP purification for downstream processing - Performed antigen detection analysis via Western Blotting and ELISA - Executed fermentation processes (Batch & Fed-batch) up to 8L scale - Coordinated external collaborations for VLP analysis (Electron Microscopy) - Managed SOP preparation, documentation, and project oversight

Functional dissection of viral RNA silencing suppressors. Expert in the recombinant expression & purification of highly challenging/difficult proteins.Expert in cloning, engineering fusion proteins (GST, HIS, SUMO), and purifying challenging proteins from E. coli (soluble & inclusion bodies) via HPLC, SEC, IEX, and affinity chromatography. Skilled in UV/Fluorescence/CD spectroscopy, EMSA, and SDM. Strong collaborator in X-ray crystallography, NMR, and mass spectrometry. Experienced mentor.

Project Title: Development of insect resistant cauliflower transgenics through Host-derived RNA interference

Title: RNAi Knockdown of Acetylcholinesterase Genes in Meloidogyne incognita for Nematode Resistance in Tobacco and Tomato Developed hairpin RNAi constructs in binary vector with 35S promoter for gene silencing. Generated transgenic tomato and tobacco plants via Agrobacterium-mediated genetic transformation. Conducted molecular characterization of transgenics using PCR, RT-PCR, Northern, and Southern blotting. Performed nematode resistance bioassays to evaluate transgenic plant resistance