Venkatesh Kumar Murugasan

Bis 2019, Research technician, Monasterium Laboratory
Münster, Deutschland

Fähigkeiten und Kenntnisse

qPCR
ELISA
HPLC
FACS Analysis
Cell Culture Technology
PCR Analysis
Cloning
qRT-PCR
AAV
transfection
Tissue Culture
SDS PAGE
Histology
Immunohistochemistry
Immunocytochemistry
Fluorescence microscopy
Western Blot
Comet assay
TUNEL Assay
MS Office
UV / Vis spectroscopy
TLC
Protein expression
Column Chromatography
Affinity Chromatography
ImageJ
GraphPad Prism

Werdegang

Berufserfahrung von Venkatesh Kumar Murugasan

  • 7 Monate, Juli 2018 - Jan. 2019

    Research technician

    Monasterium Laboratory

    • Worked in an industrial project concerning the effect of test compounds on hair growth ex-vivo using techniques such as microdissection and hair follicle organ culture, tissue processing, OCT embedding and cryosectioning, immunohistochemistry and immunofluorescence staining. • Involved in writing SOPs, lab ordering, equipment maintenance and safe disposal of hazardous wastes in accordance with German regulations.

  • 1 Jahr, Feb. 2017 - Jan. 2018

    Research assistant

    Centre for Organismal Studies, Heidelberg University

    • Actively contributed to a research project entitled “A robust and versatile homology-(in)dependent multi-fragment assembly for functional transgenesis” using various methods such as cell culture (bacteria, yeast), production of novel DNA constructs (molecular cloning), PCR and qPCR.

  • 10 Monate, Okt. 2016 - Juli 2017

    Research Intern

    Interdisciplinary Centre for Neurosciences (IZN), Heidelberg University

    • Worked on my MSc thesis entitled “Interaction between the transcriptional regulators, Tlx and Hes1 in the regulation of adult neurogenesis” using techniques such as brain dissection, cell sorting (FACS), mammalian cell culture, AAV production (Hes1 knockout), transfection, cultivation and differentiation of neural stem cells, PCR, qPCR, SDS-PAGE, immunohistochemistry and confocal microscopy.

  • 1 Jahr und 2 Monate, Sep. 2013 - Okt. 2014

    Junior Researcher

    Herbo Flora Biotech Research Centre, Coimbatore, India

    • Involved in extraction and quantification of various bioactive compounds using plant tissue culture, UV/Vis Spectroscopy, TLC and HPLC. Evaluation and documentation of results in an electronic lab book. • Responsible for drafting SOPs and conducting internal review.

  • 2 Jahre und 3 Monate, Juni 2011 - Aug. 2013

    Senior Associate- Technical Product Support

    VWR International

    • Provided excellent technical support and trouble-shooting services to the customers within North America via e-mail, live chat and hotline.

  • 1 Jahr und 5 Monate, Jan. 2010 - Mai 2011

    Scientific Assistant

    Karpagam University, Coimbatore, India

    • Worked on the research of “Expression and Purification of Human Membrane Proteins in K. lactis” using techniques such as cell culture (bacteria, yeast), PCR, molecular cloning, protein expression, SDS-PAGE, Western blot and affinity chromatography. • Involved in lab organization and equipment maintenance duties.

Ausbildung von Venkatesh Kumar Murugasan

  • 10 Monate, Okt. 2016 - Juli 2017

    MSc Biotechnology (Erasmus)

    Ruprecht-Karls-Universität Heidelberg

  • 2 Jahre und 1 Monat, Okt. 2015 - Okt. 2017

    MSc Biotechnology

    University of Rome Tor Vergata

    Gene expression and regulation, Applied immunology, Structural and Industrial biochemistry, Microbial technology, Pharmacology and Pharmaceutical chemistry, Nanobiotechnology

  • 3 Jahre und 10 Monate, Aug. 2005 - Mai 2009

    B.Tech in Industrial Biotechnology

    Government College of Technology, Coimbatore

    Cell biology, Molecular biology, Biochemistry, Microbiology, Genetic Engineering, Protein Engineering, Immunology, Enzyme Engineering, Bioprocess Engineering, Genomics and Proteomics, Molecular pathogenesis, Cancer biology, Downstream processing

Sprachen

  • Englisch

    Muttersprache

  • Deutsch

    Gut

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