mariana rodrigues

• Develop, optimize, and implement HPLC/UHPLC-MS and GC-FID/MS analytical methods for pharmaceutical raw materials, intermediates, and APIs, including potency, impurity, and stability-related analyses under CDMO contracts. • Support method validation through experimental execution, data evaluation, protocol preparation, and technical reporting in accordance with GMP/GLP requirements. • Apply Design of Experiments (DoE) and statistical approaches to analytical method development and process optimization.

•Applied advanced chromatographic and mass spectrometric methodologies for complex mixture characterization, trace-level compound identification, and analytical troubleshooting in a GLP research environment. •Developed advanced analytical methodologies combining GC-MS, GC-FID, GC-MS/O, GC-GC-MS/O, PTR-MS. •Independently operated advanced chromatographic and mass spectrometric instrumentation, including data acquisition, processing, interpretation, calibration, maintenance, and troubleshooting.

• Developed analytical extraction methodologies using Design of Experiments (DoE) to optimize compound recovery. • Conducted compound identification using UHPLC-PAD/UV, UHPLC-ESI-Q-TOF-MS, GC-MS, HPTLC, NMR, and UV-Vis. • Conducted analytical method development and validation, evaluating analytical performance and generating reliable results. • Applied green chemistry principles to analytical method development, improving analytical efficiency and sustainability.

• Developed eco-friendly extraction methodologies for bioactive compounds from coffee by-products. • Performed analytical characterization using GC-MS, UHPLC-PAD/UV, carbon-13 NMR and UV-Vis spectroscopy. • Developed an innovative extraction methodology that demonstrated improved efficiency compared with traditional extraction. • Prepared presentations, reports, and technical materials to communicate research results and scientific insights.