Andreas Altıngül

Working on recombinant MNT/MAX protein systems within the MYC/MAX/MNT transcriptional regulatory network, focusing on bacterial expression constructs, protein purification strategies, solubility and stability optimization, bHLH-LZ-mediated protein–protein interactions, and preparation of recombinant targets for aptamer-SELEX applications.

•Performed immune cell isolation and enrichment from leukapheresis material, including selection and depletion of specific cell populations. •Cell therapy manufacturing processes, including CAR-T intermediate product preparation. •Executed aseptic processing procedures in GMP-compliant cleanroom environments following SOPs, batch records, and strict documentation requirements. •Contributed to controlled manufacturing operations ensuring product quality, traceability, and regulatory compliance.

Investigating DNA aptamer–cancer cell interactions in Burkitt’s lymphoma models. Experienced in mammalian suspension cell culture (Ramos and Jurkat), aptamer preparation, cytotoxicity experiments. Skilled in treatment assays and cell viability analysis using luminescence-based methods (CellTiter-Glo). Performing flow cytometry (FACS) experiments to evaluate aptamer binding and cellular responses. Strong background in molecular biology techniques, experimental design, and laboratory data analysis.

• Designed and investigated transcription-based DNA switches for regulation of T7 RNA polymerase activity. • DNA oligonucleotide design, annealing and transcription assays to study controlled transcription systems. • Conducted fluorescence-based transcription measurements using molecular beacon. • Applied PAGE analysis, UV–Vis, and fluorescence measurements. • Performed experimental standardization, data normalization, and quality control to evaluate transcription efficiency and switching behavior.

• Conducted research in molecular biology, genetics, and biotechnology within interdisciplinary laboratory projects. • Contributed to the development of gold nanoparticle-based nanoplasmonic biosensors for biomolecular sensing. • Proto-cell construction and model membrane systems, including liposome preparation and lipid nanoparticle formation. • Experimental standardization, quality control procedures, and UV–Vis spectroscopic analysis for characterization of nanomaterials and biomolecular systems.

•Gained experience with GMP, GLP, SOP, and HSE regulations in pharmaceutical manufacturing and quality environments. •Performed and observed analytical techniques; HPLC, GC, TLC, MS, UV–Vis spec, dissolution testing, and Karl Fischer titration. •Pharmaceutical quality testing; impurity profiling, assay determination, gastro-resistance, and content uniformity tests. •Solid dosage form manufacturing processes, including mixing, granulation, compaction, tablet pressing, coating, and packaging operations.

• Conducted research on polymerization techniques with a focus on benzoxazine-based polymer systems. • Investigated strategies to suppress ring-opening polymerization (ROP) of benzoxazines to improve material stability and processing behavior. • Performed polymer characterization and quality control analyses using FT-IR spectroscopy, DSC, and TGA. • Analyzed experimental data and contributed to method optimization and research documentation.

•Performed analytical and material characterization techniques, including TGA, FT-IR, DSC, XRF, ICP, SEM, GC, and HPLC. •Conducted physicochemical characterization and analytical evaluation for R&D studies. •Participated in method standardization and analytical procedure optimization. •Analyzed experimental data and prepared technical reports on performance measurements and analytical results. •Worked according to GLP principles in a multidisciplinary research laboratory environment.

• Gas chromatography (GC), HPLC • Sample Preparation • Standardisation tests to assess compliance • GLP, GMP • Gas chromatography (GC), HPLC • Sample Preparation • Standardisation tests to assess compliance • GLP, GMP Skills: Stability Studies · Formulation · High-Performance Liquid Chromatography (HPLC) · Good Laboratory Practice (GLP) · Good Manufacturing Practice (GMP) · Analytical Method Validation · Process Validation · Time Management · Method Development · Optimization · Data Integrity · GC-MS