Dr. Denise Pedrosa

Key-staff for operating a high-speed spinning disk microscope (Nikon). Identification of disfunction and troubleshooting management, including all microscope parts. Establishment and implementation of new imaging techniques (large and small vessels, ex vivo thrombi, 3D tumor models). Highly collaborative with other teams by management and development of key in vivo experiments. Trainer of new users with focus in mouse surgery concomitant with project development and consultant for thrombosis models.

Specialist in mouse thrombosis models in real-time, from acute to chronic phase, until thrombus resolution. Use of different approaches (intravital microscopy up to 6 hours and ultrasound techniques) to study the involvement of coagulation factors expressed in monocytes during venous thrombus formation and its resolution.

Learning of acute/chronic thrombosis models in vivo with anesthesia management for intravital microscopy. The results obtained during the screening of specific mutants for coagulation factors led to a project to be developed as a Post-Doc in the same lab.

Learning of mouse handling and facility maintenance, blood collection, organs storage, pancreatic islets isolation for RNA, prepare of cryosections and histological techniques.

Human Genetics Lab (40 weekly hours). Mutation's screening of patients with congenital adrenal hyperplasia. DNA extraction and digestion with restriction enzymes, PCR and sequencing. Project developed as a bachelor thesis.

Contaminants and Residues Lab (30 weekly hours). First step in science. Validation of a method for extraction of a synthetic growth regulator (Paclobutrazol®) used in mango cultivation by liquid chromatography (HLPC). The method was highly effective for paclobutrazol determination, both in mango and soil probes, which allowed to certify a mango free of growth regulator for human consumption.

Performance of physical exercise in mice as a prophylactic intervention for thrombosis and use of hyperbaric oxygen as a treatment. The combination of both interventions showed being more effective in reducing arterial thrombosis in mice. Preparation of cryosections for identification of markers involved in thrombus resolution by immunofluorescence. Project developed concomitant with teaching of bachelor students and mentoring of a master student.

Gene expression analysis of regenerating genes in pancreatic islets of a spontaneous mouse model of diabetes type I. Full implementation of Real-Time PCR system (ExicyclerTM - Bioneer) for the research group.