Lieke Widowati
Angestellt, Research Associate, Proteros biostructures GmbH
München, Deutschland
Werdegang
Berufserfahrung von Lieke Widowati
Bis heute 3 Jahre, seit Juli 2021
Research Associate
Proteros biostructures GmbH
• Recombinant protein production in E. coli (including expression analysis) • Purification of recombinant proteins with various tags (His, GST, etc.) using diverse chromatography methods, such as affinity, ion exchange, and gel filtration chromatography • Highly trained in the use ÄKTA machines including ÄKTA Prime, Purifier, Go, and Pure (including the manual and automatic functions) • Column packing using various beads • Analysis using SDS-PAGE and Western Blot • Documentation using LIMS
1 Jahr und 4 Monate, Sep. 2018 - Dez. 2019
Research student
Martin-Luther-Universität Halle-Wittenberg
Master thesis with the title "Recombinant production of variants of Ranaspumin-2 and purification using a Car9 affinity tag" • Prepared yeast and bacteria culture (Kluyveromyces lactis, Escherichia coli) • Produced recombinant protein of Ranaspumin-2 (derived from frog foam protein of Tungara frog) • Confirmed the protein production using SDS-PAGE and Western blot. • Purified the recombinant protein using the Car9 tag using silica matrix (method: affinity chromatography) on ÄKTA pure system.
1 Jahr und 5 Monate, Dez. 2013 - Apr. 2015
Supervisor
Panen Lestari Internusa (SOGO)
11 Monate, Jan. 2013 - Nov. 2013
Technical reporter
i-Tech magazine
• Investigated and researched topics online based on trends in the field of science and technology. • Published 10 articles monthly, translating complex research conclusions for the masses (English and Indonesian) • Interviewed the university lecturers on their ongoing projects and advancements within their respective fields. Unfortunately the magazine was not well received in the market and had to be out of operation.
10 Monate, März 2012 - Dez. 2012
Product specialist
PT. Ultra Prima Abadi (Orang Tua group)
• Executed social media strategy in Facebook & Twitter, resulting in significant increases in page traffic, number of followers, and brand awareness with teen social media users. • In charge for the production of brand merchandises including keychain, fridge magnets, T-shirts, notebooks. • Assisted product manager with daily and monthly sales, also providing strategy ideas for improvement. • Completed thorough evaluations of competitors, including pricing, sales histories and marketing methods.
7 Monate, Sep. 2011 - März 2012
Management Trainee (marketing)
PT. Ultra Prima Abadi (Orang Tua group)
• Selected into a 6-month accelerated rotation program across business units (R&D, marketing, sales, manufacturing) • Monthly presentations of the progress of the final project of the position • My final project was the creation of "Giant Waffle", which was a huge replica of the snack with an automated machine that could dispense the real snacks. Two replicas were displayed in 2 prominent malls in Jakarta and was used as part of the re-launching of Tango Waffle brand.
Ausbildung von Lieke Widowati
3 Jahre und 3 Monate, Okt. 2016 - Dez. 2019
Pharmaceutical biotechnology
Martin-Luther-Universität Halle-Wittenberg
4 Jahre und 8 Monate, Aug. 2006 - März 2011
Biotechnology
Atma Jaya Catholic University of Indonesia
Sprachen
Englisch
Fließend
Deutsch
Gut
Indonesian
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