Morgane Diebold

Quality control in a pharmaceutical laboratory, in the cellular biology department. Main techniques : - Cell culture (adherent, suspension and semi-adherent cells) - In vitro bioassays - Lipofection (mRNA), protein production and extraction - ELISA - Luminescence viability assays - GMP work

Exploratory work on the understanding of atopic dermatitis mechanisms: cell-based assays to study the effect of STAT6 pathway modulations on gene expression / the interaction between STAT pathways. Main techniques: - Cell culture - RNA extraction - RT qPCR - Western blot - Immunoprecipitation - FACS - PBMCs isolation from blood (+ T cells and monocytes selection)

Development of CRISPR and shRNA tools to study the effect of genetic modulation in co-culture assays between T-cells and cancer cells. Performed techniques: - Western blot - Cell culture/co-culture - Isolation of blood PBMCs and T cells engineering - Cancer cells engineering - Gene knock-out by CRISPR - Gene knock-down by shRNA: virus production and transduction - Isolation of gDNA and PCR - FACS

Establishment of the first manipulations of a new experimental research project whose aim is to study the influence of apoptosis in gap closure dynamics on epithelial cell monolayers. Tasks performed : - Cell culture - Time lapse microscopy - Immunofluorescence assays and fluorescence microscopy - Bibliographic researches - Results presentation (PowerPoint)

Double degree in addition of engineer diploma to get extra knowledge on pharmaceutical processes (conception, development and safety regulations of drugs) and skills in bioproduction and protein purification.

Tri-national engineering school in the biotechnology field. The program is performed in English in three poles (Strasbourg, Basel and Freibourg) and provides : - Extensive knowledge and practical skills in molecular and cellular biology - Language skills in English and German - Solid scientific culture, especially in the biochemical field - Good notions in engineering, economic and social sciences.

A 2-year-university-degree with major in Biology Engineering, which provides a suitable knowledge base in biology and good laboratory skills.