Dr. Sai Prasanna Visweshwaran

Advised 23+ enterprise biotech/pharma clients on lab digitalisation, managing full project lifecycle from requirements to go-live within GMP and FDA 21 CFR Part 11 frameworks. Delivered $500K–$1M Agile projects on time/budget with 90%+ customer satisfaction Established lab IT workflows within CSV, GMP, and FDA 21 CFR Part 11 compliance frameworks Translated customer needs (URS/FDS) into LIMS/ELN configurations and validated deployments Drove adoption through training programmes, UAT, and KOL engagement

- Developed translational research project to correct muscular dystrophic mutations through homology independent target replacement by CRISPR-Cas9 nuclease - Plasmid design | Cloning | Designing sgRNAs & targeting donor or HDR cassettes | Bioinformatic analysis of sequences - iPSCs manipulation | Flow-cytometry & Flowjo | FACS analysis (Cell sorting) | Fluorescent protein imaging | primary cell culture | cell line establishment | Electroporation

- Developed a project on controlling immune cells migration with a novel anti-metastatic drug that targets highly conserved domain of an actin regulatory protein - Primary immune cell culture | Macrophage Polarization | T-cells manipulation | Dendritic cells manipulation | Cell chemotaxis | drug efficacy testing | Viral transduction (Lentiviral)

- Developed a project to follow the assembly of a multi-protein complex at the centrosomes - Established HaloTag / SnapTag system for imaging | Established inducible protein degradation system (PROTACs) | Confocal microscopy | ELISA | ELISpot | Western blot | RNAi

- Developed a project at the cross-roads of intracellular trafficking and cell migration, using tumor cell lines and patient samples - Cancer cell culture | Primary tumor tissue processing/culturing | Cytotoxicity assay | Phase contrast and fluorescence microscopy including live imaging | Various transfection methods and stable cell line creation | Cancer cell migration (2D/3D) | FACS analysis | Plasmid designing and gene cloning | Mutagenesis | Immunoprecipitation and protein purification

Affiliated doctoral school: Structure et Dynamique des Systèmes Vivants, Université Paris-Sud. Title: The role of a trimeric coiled coil protein in WASH complex assembly Thesis supervisor: Prof. Alexis Gautreau, Ecole Polytechnique, France

Cross-discipline research seminars and interactions | Lab rotations in 3 different labs focusing on different research themes related to life science Modules - Critical analysis of research articles, bibliography synthesis, scientific book review, research project development, writing and reviewing.

Bachelor's engineering degree entitled 'Genetic Engineering' Modules - Cell biology, biochemistry, microbiology, immunology, molecular biology and rDNA technology with respective laboratory and practical classes.