Dr. Shah Alam

• Designed a new research project, which is now being implemented and executed together with PhD, masters, and undergraduate students. • Manage laboratory, including buying supplies and also collaboration with other research groups for lab instruments. • In charge of the breeding of mice for cell culture and organ harvesting.

• Designed a research project for my PhD thesis, which was completed in four years with three publications. • Established protocols for primary cell culture (astrocytes from murine brain), western immunoblotting, immunostaining, and qPCR in our lab. • Planned small projects for six master and bachelor students and supervised them.

•Worked on the spin labeling of proteins with trityl spin labels. •Overexpressed, purified and spin-labeled mutants of YopO (Yersinia pestis outer membrane protein). •Used CW-EPR spectroscopy to determine the spin labelling efficiency.

• Performed screening of noncoding 6C RNAs-interacting proteins in C. glutamicum using in-vitro transcription and MALDI-TOF. • Analyzed the proteome of the FkpA- and Pup-dependent growth adaptation of C. glutamicum at different process temperatures.

Overexpressed various proteins from E. coli cells and purified them using Ni and Co affinity chromatography.

Title: Sphingosine 1-phosphate (S1P) lyase deficiency affects energy metabolism and causing astrogliosis in the murine brain. • Investigating the role of S1P in neurodegeneration • Implementing primary astrocyte culture in the current lab. • Organized laboratory work and purchase/consumables. • Maintains mouse breeding for cell culture and organ harvesting. • Supervision of bachelor and master students.

Master’s in Molecular Biotechnology, University of Bonn, Germany (Note- 1.9) Master thesis at IBG-1, Forschungszentrum Jülich. Title: Proteome analysis of the FkpA-and Pup-dependent growth adaptation of Corynebacterium glutamicum to different process temperatures • Construction of new C. glutamicum strains for protein overexpression. • Gene deletion in C. glutamicum strains to investigate the effect of gene • Performed proteome analysis of the data obtained from LC-MS/MS Curriculum Vitae