Shrivatsan Sampathkumar

- Small molecular based directed differentiation of Human iPS cells towards Cardiac lineage - Molecular cloning for Protein expression and CRISPR/Cas9 Genome editing - Genetic screening, Immunohistochemistry and Protein profiling of different cell lines - Electrophysiological parameters measurement and analysis of cardiomyocytes

Master Thesis: 'Design & Establishment of whole cell screening system for size separation using FhuA, a Membrane protein’ - Generated a co-expression platform of FhuA & Cellulase for size based separation of molecules - Optimized cloning conditions & PCR parameters for novel in-house ligase-independent cloning technique - Optimized fluorescent enzyme assays for validation of the screening system - Sub Project: Cloning, expression optimization & subsequent extraction of FhuA fusion protein

Project I: ‘In Vivo cross-linking & co-expression of membrane proteins’ - Cloned & co-expressed different membrane proteins and optimized In-vivo cross-linking procedure of EmrA-TolC & EmrB-TolC - Validated the cross-linking efficiency by protein purification & western blotting Project II: ‘Cloning & Expression of EmrR, a transcriptional regulator Protein’ - Generated protein mutants through site directed mutagenesis & optimized protein expression in different expression vectors.